Collection of 9 putative SMAD human TFs out of 12 considered proteins

DBD Alignment (Click to open/enlarge)


Click to download Alignment and Tree
No Structure and Description available
Ensembl ID HGNC symbol DBD Family Is TF? TF Assessment Binding Mode Motif Status Notes Comments
NFIA SMAD Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
NFIB SMAD Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
NFIC SMAD Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
NFIX SMAD Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SMAD1 SMAD Yes Known motif 2 Obligate heteromer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Well-established obligate heteromer
SMAD2 SMAD No Unlikely to be sequence specific TF 4 Not a DNA binding protein No motif Only known motifs are from Transfac or HocoMoco - origin is uncertain A 30 amino-acid insertion encoded by exon 3 is thought to displace the hairpin loop, providing a structural explanation for SMAD2s lack of DNA-binding activity (PMID: 11532220).
SMAD3 SMAD Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SMAD4 SMAD Yes Known motif 1 Monomer or homomultimer 100 perc ID - in vitro
SMAD5 SMAD Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SMAD6 SMAD No Unlikely to be sequence specific TF 4 Not a DNA binding protein No motif Only known motifs are from Transfac or HocoMoco - origin is uncertain In the inhibitory Smads, Smad6 and Smad7 and DAD, the MH1 domains are replaced by divergent amino-termini that share regions of similarity within the inhibitory Smad subgroup (PMID: 11532220).
SMAD7 SMAD No Unlikely to be sequence specific TF 4 Not a DNA binding protein No motif Only known motifs are from Transfac or HocoMoco - origin is uncertain In the inhibitory Smads, Smad6 and Smad7 and DAD, the MH1 domains are replaced by divergent amino-termini that share regions of similarity within the inhibitory Smad subgroup (PMID: 11532220).
SMAD9 SMAD Yes Known motif 2 Obligate heteromer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain