Collection of 8 putative RFX human TFs out of 8 considered proteins

DBD Alignment (Click to open/enlarge)


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Description
The RFX-family has 7 members in human, and as FOX and ETS TFs, its DBD is based on a winged helix-turn-helix fold. However, RFXs have evolved a different strategy for binding the DNA and recognizes different sequences. RFX DBDs can bind DNA as monomers or homodimers73,130. The site bound most strongly is GTTGCCATGGCAAC, but these proteins can also recognize other binding configurations, the most relevant of which are the one and two bases shorter spacing variants of the same palindromic configuration. The structure of the RFX binding to its canonical palindromic target site has been solved using X-ray crystallography. In this structure two copies of RFX bind to opposite sides of the DNA without contacts between the DBDs. This suggests that the TF uses a close DNA allostery based mechanism as basis for its recognition of the dimeric target sites 		Structure
Ensembl ID HGNC symbol DBD Family Is TF? TF Assessment Binding Mode Motif Status Notes Comments
RFX1 RFX Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
RFX2 RFX Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
RFX3 RFX Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
RFX4 RFX Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
RFX5 RFX Yes Known motif 1 Monomer or homomultimer High-throughput in vitro Has a putative AT-hook
RFX6 RFX Yes Inferred motif 1 Monomer or homomultimer High-throughput in vitro
RFX7 RFX Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
RFX8 RFX Yes Likely to be sequence specific TF 1 Monomer or homomultimer No motif RFX8 has the HUGO-approved name RFX family member 8, lacking RFX DNA binding domain?.? I cannot find the original evidence, however, and RFX8 clearly encodes a complete and high-confidence RFX DNA binding domain.? So, it seems more likely than not to be DNA binding (TRH)