TF Info Page for SPIB (Ets)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Spi-B transcription factor [Source:HGNC Symbol;Acc:HGNC:11242]
Entrez Summary The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Ensembl ID: ENSG00000269404
External Link: CisBP
Interpro IPR000418; IPR006134; IPR025687; ;
Protein Domain: ENSP00000270632
Protein Domain: ENSP00000471921
Protein Domain: ENSP00000391877
Protein Domain: ENSP00000470970
Protein Domain: ENSP00000472626
Domain:
Protein: ENSP00000270632DBD: ETSOther:
Protein: ENSP00000471921DBD: ETSOther:
Protein: ENSP00000391877DBD: ETSOther:
Protein: ENSP00000470970DBD: ETSOther:
Protein: ENSP00000472626DBD: ETSOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:1406622
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
SELEXJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERDirect
HT-SELEXYin2017Inferred - SPIB (100% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - SPIB (100% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SPIB (100% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SPIB (100% AA Identity, Homo sapiens)
SELEXJASPARInferred - SPIB (100% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SPIB (100% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SPIB (100% AA Identity, Homo sapiens)
MiscHocoMocoInferred - CTD-2545M3.6 (100% AA Identity, Homo sapiens)
MiscHOMERInferred - SPIB (100% AA Identity, Homo sapiens)
PBMBadis09Inferred - Spi1 (73% AA Identity, Mus musculus)
PBMWei10Inferred - Spi1 (73% AA Identity, Mus musculus)
ChIP-seqENCODEInferred - SPI1 (73% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - SPI1 (73% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - SPI1 (73% AA Identity, Homo sapiens)
ChIP-seqhmCHIPInferred - SPI1 (73% AA Identity, Homo sapiens)
SELEXJASPARInferred - SPI1 (73% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SPI1 (73% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SPI1 (73% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SPI1 (73% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SPI1 (73% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SPI1 (73% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SPI1 (73% AA Identity, Homo sapiens)
ChIP-seqhmCHIPInferred - Spi1 (73% AA Identity, Mus musculus)
MiscHocoMocoInferred - SPI1 (73% AA Identity, Homo sapiens)
MiscHOMERInferred - Spi1 (73% AA Identity, Mus musculus)
PBMWei10Inferred - Spic (61% AA Identity, Mus musculus)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution