TF Info Page for CEBPA (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer 100 perc ID - in vitro

Description

Description: CCAAT/enhancer binding protein alpha [Source:HGNC Symbol;Acc:HGNC:1833]
Entrez Summary This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]
Ensembl ID: ENSG00000245848
External Link: CisBP
Interpro IPR004827; IPR016468;
Protein Domain: ENSP00000427514
Domain:
Protein: ENSP00000427514DBD: bZIPOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_Transactivation_
PMIDS:2227417 8632009
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
No
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IMP - PMID:16946298
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:11242107, PMID:12695546, PMID:15664994
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
NAS - PMID:7575576
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
ChIP-seqJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMMann2013Inferred - Cebpa (100% AA Identity, Mus musculus)
PBMMann2013Inferred - Cebpa (100% AA Identity, Mus musculus)
PBMZoo_01Inferred - Cebpa (100% AA Identity, Mus musculus)
PBMDREAM_contestInferred - Cebpb (73% AA Identity, Mus musculus)
PBMMann2013Inferred - Cebpb (73% AA Identity, Mus musculus)
PBMMann2013Inferred - Cebpb (73% AA Identity, Mus musculus)
PBMZoo_01Inferred - Cebpb (73% AA Identity, Mus musculus)
MiscHOMERInferred - Cebpb (73% AA Identity, Mus musculus)
SMiLE-seqIsakova2017Inferred - CEBPB (72% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - CEBPB (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - CEBPB (72% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - CEBPB (72% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - CEBPB (72% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - CEBPB (72% AA Identity, Homo sapiens)
SELEXJASPARInferred - CEBPB (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPB (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPB (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPB (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPB (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPB (72% AA Identity, Homo sapiens)
MiscHocoMocoInferred - CEBPB (72% AA Identity, Homo sapiens)
HT-SELEXJolma2010Inferred - CEBPE (71% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - CEBPE (71% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - CEBPE (71% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPE (71% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPE (71% AA Identity, Homo sapiens)
MiscHocoMocoInferred - CEBPE (71% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Cebpe (69% AA Identity, Mus musculus)
PBMMann2013Inferred - BAE29047.1 (66% AA Identity, Mus musculus)
PBMMann2013Inferred - BAE29047.1 (66% AA Identity, Mus musculus)
PBMZoo_01Inferred - BAE29047.1 (66% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - CEBPD (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - CEBPD (66% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPD (66% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPD (66% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CEBPD (66% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Cebpd (66% AA Identity, Rattus norvegicus)
MiscHocoMocoInferred - CEBPD (66% AA Identity, Homo sapiens)

Structure

Structure PDB 1NWQ

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution