The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

ETS variant 5 [Source:HGNC Symbol;Acc:HGNC:3494]

Entrez Summary

Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in cellular response to oxidative stress; negative regulation of transcription by RNA polymerase II; and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022]

Ensembl ID:

ENSG00000244405

External Link:

CisBP

Interpro

IPR000418; IPR006715;

Protein Domain:

ENSP00000306894

Protein Domain:

ENSP00000413755

Protein Domain:

ENSP00000412171

Domain:

Protein: ENSP00000306894	DBD: ETS	Other: ETS_PEA3_N
Protein: ENSP00000413755	DBD: ETS	Other: ETS_PEA3_N
Protein: ENSP00000412171	DBD: ETS	Other: ETS_PEA3_N

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:15138262 15466854 15857832
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001228 RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor IMP - PMID:15857832 GO:0003700 sequence-specific DNA binding transcription factor activity IDA - PMID:19443906
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Misc	HocoMoco		Direct
PBM	Wei10		Inferred - Etv5 (100% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - ETV1 (96% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ETV1 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ETV1 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ETV1 (96% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Etv1 (96% AA Identity, Mus musculus)
PBM	Wei10		Inferred - Etv1 (96% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - ETV4 (96% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ETV4 (96% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ETV4 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ETV4 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ETV4 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ETV4 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ETV4 (96% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Etv4 (96% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - ETV4 (96% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ETV4 (96% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - ETV1 (96% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - ETV4 (96% AA Identity, Homo sapiens)
HT-SELEX	Nitta2015		Inferred - Ets96B (87% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - pnt (65% AA Identity, Drosophila melanogaster)
PBM	Wei10		Inferred - Ets1 (64% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - ETS1B_CHICK (64% AA Identity, Gallus gallus)
PBM	Wei10		Inferred - Elk4 (61% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - ELK4 (60% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELK4 (60% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELK4 (60% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - ELK4 (60% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - ELK4 (60% AA Identity, Homo sapiens)
ChIP-seq	JASPAR		Inferred - ELK4 (60% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELK4 (60% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELK4 (60% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - ELK4 (60% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELK1 (59% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELK1 (59% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELK1 (59% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELK1 (59% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Elk1 (59% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - FEV (59% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FEV (59% AA Identity, Homo sapiens)
SELEX	JASPAR		Inferred - ELK1 (59% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELK1 (59% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELK1 (59% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELK1 (59% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELK1 (59% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELK1 (59% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - ELK1 (59% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - ELK1 (59% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - FEV (59% AA Identity, Homo sapiens)
HT-SELEX	Nitta2015		Inferred - Ets21C (58% AA Identity, Drosophila melanogaster)
PBM	Zoo_01		Inferred - Ets21C (58% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - Ets65A (58% AA Identity, Drosophila melanogaster)
SMiLE-seq	Isakova2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FLI1 (58% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Fli1 (58% AA Identity, Mus musculus)
SELEX	JASPAR		Inferred - FLI1 (58% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - FLI1 (58% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - FLI1 (58% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - FLI1 (58% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - Fli1 (58% AA Identity, Mus musculus)
PBM	Zoo_01		Inferred - elk4 (57% AA Identity, Tetraodon nigroviridis)
HT-SELEX	Yin2017		Inferred - ELK3 (56% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELK3 (56% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELK3 (56% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELK3 (56% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELK3 (56% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELK3 (56% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Elk3 (56% AA Identity, Mus musculus)
SMiLE-seq	Isakova2017		Inferred - GABPA (56% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - GABPA (56% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - GABPA (56% AA Identity, Homo sapiens)
PBM	Badis09		Inferred - Gabpa (56% AA Identity, Mus musculus)
PBM	Wei10		Inferred - Gabpa (56% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - ELK3 (56% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - GABPA (56% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - GABPA (56% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - GABPA (56% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - GABPA (56% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - GABPA (56% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - GABPA (56% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - GABPA (56% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - GABPA (56% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - GABPA (56% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - GABPA (56% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - GABPA (56% AA Identity, Homo sapiens)
ChIP-seq	JASPAR		Inferred - Gabpa (56% AA Identity, Mus musculus)
Misc	HocoMoco		Inferred - ELK3 (56% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - GABPA (56% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - GABPA (56% AA Identity, Homo sapiens)
HT-SELEX	Jolma2010		Inferred - ERG (55% AA Identity, Homo sapiens)
HT-SELEX	Jolma2010		Inferred - ERG (55% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ERG (55% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ERG (55% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ERG (55% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ERG (55% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ERG (55% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ERG (55% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ERG (55% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ERG (55% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Erg (55% AA Identity, Mus musculus)
SELEX	JASPAR		Inferred - ERG (55% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ERG (55% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ERG (55% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - ERG (55% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - ERG (55% AA Identity, Homo sapiens)

Structure

Structure	PDB	4UNO

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 448, "endStyle": "curved", "aliStart": 369, "text": "Ets", "colour": "#009900", "aliEnd": 448, "start": 369, "href": "http://pfam.xfam.org/family/PF00178.20", "type": "pfama", "display": "true", "metadata": {"end": 448, "description": "The ETS domain is a winged helix-turn-helix structure that binds to DNA sites with a central GGA(A/T) DNA sequence. ", "database": "PfamA", "aliStart": 369, "scoreName": "E-value", "accession": "PF00178.20", "start": 369, "score": 5.799999999999999e-38, "identifier": "Ets-domain", "type": "DBD", "aliEnd": 448}}, {"startStyle": "straight", "end": 366, "endStyle": "straight", "aliStart": 1, "text": "ETS_PEA3_N", "colour": "#9999ff", "aliEnd": 366, "start": 1, "href": "http://pfam.xfam.org/family/PF04621.11", "type": "pfama", "display": "true", "metadata": {"end": 366, "description": "The N terminus of the PEA3 transcription factors is implicated in transactivation and in inhibition of DNA binding [1]. Transactivation is potentiated by activation of the Ras/MAP kinase and protein kinase A signalling cascades.  The N terminal region contains conserved MAP kinase phosphorylation sites [2].", "database": "PfamA", "aliStart": 1, "scoreName": "E-value", "accession": "PF04621.11", "start": 1, "score": 1.7999999999999996e-162, "identifier": "PEA3 subfamily ETS-domain transcription factor N terminal domain", "type": "DBD", "aliEnd": 366}}], "length": 511}

TF Info Page for ETV5 (Ets)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution