TF Info Page for EBF2 (EBF1)


Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain EBF1 has a nice HT-SELEX model and based on this paper (PMID:23499423). EBF2 binds similar motif to control brown/beige fat cell identity.


Description: early B-cell factor 2 [Source:HGNC Symbol;Acc:HGNC:19090]
Entrez Summary The protein encoded by this gene belongs to the COE (Collier/Olf/EBF) family of non-basic, helix-loop-helix transcription factors that have a well conserved DNA binding domain. The COE family proteins play an important role in variety of developmental processes. Studies in mouse suggest that this gene may be involved in the differentiation of osteoblasts. [provided by RefSeq, Oct 2011]
Ensembl ID: ENSG00000221818
External Link: CisBP
Interpro IPR002909; IPR014756; IPR018350; ;
Protein Domain: ENSP00000386178
Protein Domain: ENSP00000430241
Protein Domain: ENSP00000437909
Protein: ENSP00000386178DBD: COE1 DBDOther: COE1_HLH, TIG
Protein: ENSP00000430241DBD: COE1 DBDOther: COE1_HLH, TIG
Protein: ENSP00000437909DBD: COE1 DBDOther: COE1_HLH

Previous Annotations

Source Annotation
TF-CAT classification No
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements


Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredInferred - Ebf2 (99% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - EBF3 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Ebf1 (82% AA Identity, Mus musculus)
MiscHOMERInferred - Ebf1 (82% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - EBF4 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Ebf4 (79% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - kn (70% AA Identity, Drosophila melanogaster)


Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution