TF Info Page for HMX1 (Homeodomain)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: H6 family homeobox 1 [Source:HGNC Symbol;Acc:HGNC:5017]
Entrez Summary This gene encodes a transcription factor that belongs to the H6 family of homeobox proteins. This protein can bind a 5'-CAAG-3' core DNA sequence, and it is involved in the development of craniofacial structures. Mutations in this gene cause oculoauricular syndrome, a disorder of the eye and external ear. [provided by RefSeq, Oct 2009]
Ensembl ID: ENSG00000215612
External Link: CisBP
Interpro IPR001356; IPR009057; IPR017970; IPR020479;
Protein Domain: ENSP00000383516
Protein Domain: ENSP00000446997
Domain:
Protein: ENSP00000383516DBD: HomeodomainOther:
Protein: ENSP00000446997DBD: HomeodomainOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info GO:0001227
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
PBMBerger08Inferred - Hmx1 (100% AA Identity, Mus musculus)
B1HJASPARInferred - Hmx (94% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Hmx (94% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - Hmx3 (94% AA Identity, Mus musculus)
PBMBerger08Inferred - Hmx3_CONSTRUCT (91% AA Identity, PBM CONSTRUCTS)
HT-SELEXYin2017Inferred - HMX2 (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HMX2 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HMX2 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HMX2 (89% AA Identity, Homo sapiens)
PBMBerger08Inferred - Hmx2 (89% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - NKX3-2 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NKX3-2 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NKX3-2 (68% AA Identity, Homo sapiens)
PBMBerger08Inferred - Nkx3-2 (68% AA Identity, Mus musculus)
SELEXJASPARInferred - NKX3-2 (68% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NKX3-2 (68% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NKX3-2 (68% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution