TF Info Page for MEF2B (MADS box)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: myocyte enhancer factor 2B [Source:HGNC Symbol;Acc:HGNC:6995]
Entrez Summary The product of this gene is a member of the MADS/MEF2 family of DNA binding proteins. The protein is thought to regulate gene expression, including expression of the smooth muscle myosin heavy chain gene. This region undergoes considerable alternative splicing, with transcripts supporting two non-overlapping loci (GeneID 729991 and 100271849) as well as numerous read-through transcripts that span both loci (annotated as GeneID 4207). Several isoforms of this protein are expressed from either this locus or from some of the read-through transcripts annotated on GeneID 4207. [provided by RefSeq, Jan 2014]
Ensembl ID: ENSG00000213999
External Link: CisBP
Interpro IPR002100; ;
Protein Domain: ENSP00000386480
Protein Domain: ENSP00000386784
Protein Domain: ENSP00000386374
Protein Domain: ENSP00000402154
Domain:
Protein: ENSP00000386480DBD: MADS boxOther:
Protein: ENSP00000386784DBD: MADS boxOther:
Protein: ENSP00000386374DBD: MADS boxOther:
Protein: ENSP00000402154DBD: MADS boxOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:7760790
GO:0003700
sequence-specific DNA binding transcription factor activity
NAS - PMID:8575763
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredInferred - Mef2b (95% AA Identity, Mus musculus)
HT-SELEXJolma2010Inferred - MEF2C (91% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MEF2C (91% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MEF2C (91% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - MEF2A (91% AA Identity, Homo sapiens)
SELEXJASPARInferred - MEF2A (91% AA Identity, Homo sapiens)
ChIP-seqmodENCODEInferred - MEF2A (91% AA Identity, Homo sapiens)
ChIP-seqmodENCODEInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2A (91% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - MEF2C (91% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - MEF2C (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2C (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2C (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2C (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2C (91% AA Identity, Homo sapiens)
MiscHocoMocoInferred - MEF2A (91% AA Identity, Homo sapiens)
MiscHOMERInferred - Mef2a (91% AA Identity, Mus musculus)
MiscHocoMocoInferred - MEF2C (91% AA Identity, Homo sapiens)
MiscHOMERInferred - MEF2C (91% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MEF2D (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MEF2D (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2D (89% AA Identity, Homo sapiens)
MiscHocoMocoInferred - MEF2D (89% AA Identity, Homo sapiens)
ChIP-seqmodENCODEInferred - Mef2 (83% AA Identity, Drosophila melanogaster)

Structure

Structure PDB 1N6J

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution