TF Info Page for E2F4 (E2F)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: E2F transcription factor 4 [Source:HGNC Symbol;Acc:HGNC:3118]
Entrez Summary NA
Ensembl ID: ENSG00000205250
External Link: CisBP
Interpro IPR003316; ;
Protein Domain: ENSP00000368686
Protein Domain: ENSP00000458706
Domain:
Protein: ENSP00000368686DBD: E2FOther: E2F_CC-MB
Protein: ENSP00000458706DBD: E2FOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific
Transcription Factor Binding
tf co-factor binding_DNA Binding
Transactivation_
PMIDS:12110166
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IC - GO_REF:0000036
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:11418595
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERDirect
MiscHocoMocoInferred - E2F5 (75% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - efl-1 (66% AA Identity, Caenorhabditis elegans)
ChIP-seqmodENCODEInferred - efl-1 (66% AA Identity, Caenorhabditis elegans)
MiscHOMERInferred - efl-1 (66% AA Identity, Caenorhabditis elegans)

Structure

Structure PDB 1CF7

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution