TF Info Page for MAFK (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: MAF bZIP transcription factor K [Source:HGNC Symbol;Acc:HGNC:6782]
Entrez Summary The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008]
Ensembl ID: ENSG00000198517
External Link: CisBP
Interpro IPR004826; IPR004827; IPR008917; ;
Protein Domain: ENSP00000344903
Protein Domain: ENSP00000385437
Domain:
Protein: ENSP00000344903DBD: bZIPOther:
Protein: ENSP00000385437DBD: bZIPOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Other
Transactivation_
PMIDS:11013233 14960151
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IBA - GO_REF:0000033
GO:0003700
sequence-specific DNA binding transcription factor activity
NAS - PMID:9150357
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - Mafk (100% AA Identity, Mus musculus)
MiscHOMERInferred - Mafk (100% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFG (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MAFG (85% AA Identity, Homo sapiens)
MiscHocoMocoInferred - MAFG (85% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Mafg (84% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - MAFF (78% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MAFF (78% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MAFF (78% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MAFF (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFF (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFF (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFF (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MAFF (78% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - MAFF (78% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - MAFF (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MAFF (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MAFF (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Maff (78% AA Identity, Mus musculus)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution