The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Inferred motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

hes family bHLH transcription factor 4 [Source:HGNC Symbol;Acc:HGNC:24149]

Entrez Summary

Ensembl ID:

ENSG00000188290

External Link:

CisBP

Interpro

IPR003650; IPR011598;

Protein Domain:

ENSP00000304595

Protein Domain:

ENSP00000393198

Protein Domain:

ENSP00000425085

Domain:

Protein: ENSP00000304595	DBD: bHLH	Other: Hairy_orange
Protein: ENSP00000393198	DBD: bHLH	Other: Hairy_orange
Protein: ENSP00000425085	DBD: bHLH	Other: Hairy_orange

Previous Annotations

Source	Annotation
TF-CAT classification	No PMIDS:
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	b
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	No
GO-Info
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a2, Orthologous HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Yin2017		Inferred - HES1 (87% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - HES1 (87% AA Identity, Homo sapiens)
PBM	Zoo_01		Inferred - Hes1 (87% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - HES1 (87% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - HES1 (87% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - HES1 (87% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - HES1 (87% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - HES1 (87% AA Identity, Homo sapiens)
B1H	JASPAR		Inferred - h (70% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - h (70% AA Identity, Drosophila melanogaster)
Transfac	Transfac	License required	Inferred - h (70% AA Identity, Drosophila melanogaster)
Misc	iDMMPMM		Inferred - h (70% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - dpn (67% AA Identity, Drosophila melanogaster)
HT-SELEX	Yin2017		Inferred - HES2 (67% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - HES2 (67% AA Identity, Homo sapiens)
PBM	Zoo_01		Inferred - Hes2 (67% AA Identity, Mus musculus)
HT-SELEX	Nitta2015		Inferred - Side (53% AA Identity, Drosophila melanogaster)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for HES4 (bHLH)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution