TF Info Page for ASCL4 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Inferred motif 1 Monomer or homomultimer High-throughput in vitro Probably heterodimerizes with TCF3; TCF4 or TCF12 like the other ASCL TFs

Description

Description: Achaete-scute family bHLH transcription factor 4 [Source:HGNC Symbol;Acc:HGNC:24311]
Entrez Summary Basic helix-loop-helix transcription factors, such as ASCL4, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM, Mar 2008]
Ensembl ID: ENSG00000187855
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000345420
Domain:
Protein: ENSP00000345420DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
3a, decent circumstantial evidence for its role as
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Inferred - ASCL1 (54% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ASCL1 (54% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ASCL2 (54% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ASCL2 (54% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ASCL2 (54% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ASCL2 (54% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ASCL2 (54% AA Identity, Homo sapiens)
PBMBadis09Inferred - Ascl2 (54% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ASCL1 (54% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ASCL1 (54% AA Identity, Homo sapiens)
MiscHOMERInferred - Ascl1 (54% AA Identity, Mus musculus)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution