TF Info Page for SP1 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Sp1 transcription factor [Source:HGNC Symbol;Acc:HGNC:11205]
Entrez Summary The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
Ensembl ID: ENSG00000185591
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000329357
Protein Domain: ENSP00000404263
Protein Domain: ENSP00000458133
Protein Domain: ENSP00000457804
Domain:
Protein: ENSP00000329357DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000404263DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000458133DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000457804DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:15282343 15589828
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000982
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:21659522
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:12169688, PMID:12560508
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
ChIP-seqJASPARDirect
ChIP-seqSchmitges2016Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHocoMocoDirect
PBMDREAM_contestInferred - Sp1 (98% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - SP3 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP3 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP3 (89% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP3 (89% AA Identity, Homo sapiens)
PBMLam11Inferred - ZFA5_CONSTRUCT (87% AA Identity, PBM CONSTRUCTS)
ChIP-seqSchmitges2016Inferred - SP4 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP4 (87% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP4 (87% AA Identity, Homo sapiens)
PBMBadis09Inferred - Sp4 (86% AA Identity, Mus musculus)
SMiLE-seqIsakova2017Inferred - Sp4 (86% AA Identity, Mus musculus)
PBMZoo_01Inferred - Sp4 (86% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - SP9 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP9 (86% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - SP8 (84% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP8 (84% AA Identity, Homo sapiens)
PBMLam11Inferred - ZFA93_CONSTRUCT (84% AA Identity, PBM CONSTRUCTS)
HT-SELEXNitta2015Inferred - Sp1 (83% AA Identity, Drosophila melanogaster)
PBMLam11Inferred - ZFA4_CONSTRUCT (83% AA Identity, PBM CONSTRUCTS)
TransfacTransfacLicense requiredInferred - SP5 (82% AA Identity, Homo sapiens)
PBMLam11Inferred - ZFA3_CONSTRUCT (78% AA Identity, PBM CONSTRUCTS)
TransfacTransfacLicense requiredInferred - SP7 (76% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - SP2 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP2 (72% AA Identity, Homo sapiens)
PBMCEPDInferred - sptf-3 (72% AA Identity, Caenorhabditis elegans)
ChIP-seqENCODEInferred - SP2 (72% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - SP2 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (72% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP2 (72% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution