The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

interferon regulatory factor 7 [Source:HGNC Symbol;Acc:HGNC:6122]

Entrez Summary

This gene encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. It has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. The encoded protein plays an important role in the innate immune response against DNA and RNA viruses. [provided by RefSeq, Jul 2021]

Ensembl ID:

ENSG00000185507

External Link:

CisBP

Interpro

IPR001346; IPR008984; IPR019471; IPR019817;

Protein Domain:

ENSP00000331803

Protein Domain:

ENSP00000380697

Protein Domain:

ENSP00000380700

Protein Domain:

ENSP00000380704

Domain:

Protein: ENSP00000331803	DBD: Interferon regulatory factor (IRF)	Other: IRF-3
Protein: ENSP00000380697	DBD: Interferon regulatory factor (IRF)	Other: IRF-3
Protein: ENSP00000380700	DBD: Interferon regulatory factor (IRF)	Other: IRF-3
Protein: ENSP00000380704	DBD: Interferon regulatory factor (IRF)	Other: IRF-3

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding Transactivation_ PMIDS:9786932
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0000982 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IDA - PMID:17404045
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2013		Direct
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
HT-SELEX	Yin2017		Inferred - IRF7 (100% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - IRF7 (100% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ENSG00000276561 (100% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ENSG00000276561 (100% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ENSG00000276561 (100% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - IRF7 (100% AA Identity, Homo sapiens)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 125, "endStyle": "curved", "aliStart": 14, "text": "IRF", "colour": "#009900", "aliEnd": 124, "start": 13, "href": "http://pfam.xfam.org/family/PF00605.15", "type": "pfama", "display": "true", "metadata": {"end": 125, "description": "This family of transcription factors are important in the regulation of interferons in response to infection by virus and in the regulation of interferon-inducible genes. Three of the five conserved tryptophan residues bind to DNA.", "database": "PfamA", "aliStart": 14, "scoreName": "E-value", "accession": "PF00605.15", "start": 13, "score": 4.799999999999999e-38, "identifier": "Interferon regulatory factor transcription factor", "type": "DBD", "aliEnd": 124}}, {"startStyle": "straight", "end": 437, "endStyle": "straight", "aliStart": 260, "text": "IRF-3", "colour": "#9999ff", "aliEnd": 434, "start": 258, "href": "http://pfam.xfam.org/family/PF10401.7", "type": "pfama", "display": "true", "metadata": {"end": 437, "description": "This is the interferon-regulatory factor 3 chain of the hetero-dimeric structure which also contains the shorter chain CREB-binding protein.  These two subunits make up the DRAF1 (double-stranded RNA-activated factor 1).          Viral dsRNA produced during viral transcription or replication leads to the activation of DRAF1. The DNA-binding specificity of DRAF1 correlates with transcriptional induction of ISG (interferon-alpha,beta-stimulated gene). IRF-3 preexists in the cytoplasm of uninfected cells and translocates to the nucleus following viral infection. Translocation of IRF-3 is accompanied by an increase in serine and threonine phosphorylation, and association with the CREB coactivator occurs only after infection.", "database": "PfamA", "aliStart": 260, "scoreName": "E-value", "accession": "PF10401.7", "start": 258, "score": 1.6999999999999998e-47, "identifier": "Interferon-regulatory factor 3", "type": "DBD", "aliEnd": 434}}], "length": 475}

{"regions": [{"startStyle": "curved", "end": 138, "endStyle": "curved", "aliStart": 27, "text": "IRF", "colour": "#009900", "aliEnd": 137, "start": 26, "href": "http://pfam.xfam.org/family/PF00605.15", "type": "pfama", "display": "true", "metadata": {"end": 138, "description": "This family of transcription factors are important in the regulation of interferons in response to infection by virus and in the regulation of interferon-inducible genes. Three of the five conserved tryptophan residues bind to DNA.", "database": "PfamA", "aliStart": 27, "scoreName": "E-value", "accession": "PF00605.15", "start": 26, "score": 5.7e-38, "identifier": "Interferon regulatory factor transcription factor", "type": "DBD", "aliEnd": 137}}, {"startStyle": "straight", "end": 479, "endStyle": "straight", "aliStart": 302, "text": "IRF-3", "colour": "#9999ff", "aliEnd": 476, "start": 300, "href": "http://pfam.xfam.org/family/PF10401.7", "type": "pfama", "display": "true", "metadata": {"end": 479, "description": "This is the interferon-regulatory factor 3 chain of the hetero-dimeric structure which also contains the shorter chain CREB-binding protein.  These two subunits make up the DRAF1 (double-stranded RNA-activated factor 1).          Viral dsRNA produced during viral transcription or replication leads to the activation of DRAF1. The DNA-binding specificity of DRAF1 correlates with transcriptional induction of ISG (interferon-alpha,beta-stimulated gene). IRF-3 preexists in the cytoplasm of uninfected cells and translocates to the nucleus following viral infection. Translocation of IRF-3 is accompanied by an increase in serine and threonine phosphorylation, and association with the CREB coactivator occurs only after infection.", "database": "PfamA", "aliStart": 302, "scoreName": "E-value", "accession": "PF10401.7", "start": 300, "score": 2.0999999999999996e-47, "identifier": "Interferon-regulatory factor 3", "type": "DBD", "aliEnd": 476}}], "length": 517}

{"regions": [{"startStyle": "curved", "end": 138, "endStyle": "curved", "aliStart": 27, "text": "IRF", "colour": "#009900", "aliEnd": 137, "start": 26, "href": "http://pfam.xfam.org/family/PF00605.15", "type": "pfama", "display": "true", "metadata": {"end": 138, "description": "This family of transcription factors are important in the regulation of interferons in response to infection by virus and in the regulation of interferon-inducible genes. Three of the five conserved tryptophan residues bind to DNA.", "database": "PfamA", "aliStart": 27, "scoreName": "E-value", "accession": "PF00605.15", "start": 26, "score": 5.099999999999999e-38, "identifier": "Interferon regulatory factor transcription factor", "type": "DBD", "aliEnd": 137}}, {"startStyle": "straight", "end": 450, "endStyle": "straight", "aliStart": 273, "text": "IRF-3", "colour": "#9999ff", "aliEnd": 447, "start": 271, "href": "http://pfam.xfam.org/family/PF10401.7", "type": "pfama", "display": "true", "metadata": {"end": 450, "description": "This is the interferon-regulatory factor 3 chain of the hetero-dimeric structure which also contains the shorter chain CREB-binding protein.  These two subunits make up the DRAF1 (double-stranded RNA-activated factor 1).          Viral dsRNA produced during viral transcription or replication leads to the activation of DRAF1. The DNA-binding specificity of DRAF1 correlates with transcriptional induction of ISG (interferon-alpha,beta-stimulated gene). IRF-3 preexists in the cytoplasm of uninfected cells and translocates to the nucleus following viral infection. Translocation of IRF-3 is accompanied by an increase in serine and threonine phosphorylation, and association with the CREB coactivator occurs only after infection.", "database": "PfamA", "aliStart": 273, "scoreName": "E-value", "accession": "PF10401.7", "start": 271, "score": 1.8e-47, "identifier": "Interferon-regulatory factor 3", "type": "DBD", "aliEnd": 447}}], "length": 488}

{"regions": [{"startStyle": "curved", "end": 125, "endStyle": "curved", "aliStart": 14, "text": "IRF", "colour": "#009900", "aliEnd": 124, "start": 13, "href": "http://pfam.xfam.org/family/PF00605.15", "type": "pfama", "display": "true", "metadata": {"end": 125, "description": "This family of transcription factors are important in the regulation of interferons in response to infection by virus and in the regulation of interferon-inducible genes. Three of the five conserved tryptophan residues bind to DNA.", "database": "PfamA", "aliStart": 14, "scoreName": "E-value", "accession": "PF00605.15", "start": 13, "score": 5.4e-38, "identifier": "Interferon regulatory factor transcription factor", "type": "DBD", "aliEnd": 124}}, {"startStyle": "straight", "end": 466, "endStyle": "straight", "aliStart": 289, "text": "IRF-3", "colour": "#9999ff", "aliEnd": 463, "start": 287, "href": "http://pfam.xfam.org/family/PF10401.7", "type": "pfama", "display": "true", "metadata": {"end": 466, "description": "This is the interferon-regulatory factor 3 chain of the hetero-dimeric structure which also contains the shorter chain CREB-binding protein.  These two subunits make up the DRAF1 (double-stranded RNA-activated factor 1).          Viral dsRNA produced during viral transcription or replication leads to the activation of DRAF1. The DNA-binding specificity of DRAF1 correlates with transcriptional induction of ISG (interferon-alpha,beta-stimulated gene). IRF-3 preexists in the cytoplasm of uninfected cells and translocates to the nucleus following viral infection. Translocation of IRF-3 is accompanied by an increase in serine and threonine phosphorylation, and association with the CREB coactivator occurs only after infection.", "database": "PfamA", "aliStart": 289, "scoreName": "E-value", "accession": "PF10401.7", "start": 287, "score": 1.9999999999999997e-47, "identifier": "Interferon-regulatory factor 3", "type": "DBD", "aliEnd": 463}}], "length": 504}

TF Info Page for IRF7 (IRF)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution