The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	heat shock transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:5224]
Entrez Summary	TBA
Ensembl ID:	ENSG00000185122
External Link:
Interpro	IPR000232; IPR010542;
Protein Domain:
Protein: ENSP00000431512	DBD: HSF	Other: Vert_HS_TF
Protein: ENSP00000383590	DBD: HSF	Other: Vert_HS_TF
Protein: ENSP00000436616	DBD: HSF	Other: HMMR_C, ZapB

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:9545528
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001078 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IDA - PMID:8926278 GO:0003700 sequence-specific DNA binding transcription factor activity TAS - PMID:10821850, PMID:1871105
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2013		Direct
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
ChIP-seq	ENCODE		Direct
SELEX	JASPAR		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
Misc	HOMER		Direct
Misc	HOMER		Inferred - Hsf1 (100% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - HSF4 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - HSF4 (76% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - HSF4 (76% AA Identity, Homo sapiens)

Structure

Structure	PDB	5D5U

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 118, "endStyle": "curved", "aliStart": 18, "text": "HSF", "colour": "#009900", "aliEnd": 118, "start": 18, "href": "http://pfam.xfam.org/family/PF00447.15", "type": "pfama", "display": "true", "metadata": {"end": 118, "description": "Heat shock factor (HSF) is a transcriptional activator of heat shock genes [PUBMED:2257625]: it binds specifically to heat shock promoter elements, which are palindromic sequences rich with repetitive purine and pyrimidine motifs [PUBMED:2257625].", "database": "PfamA", "aliStart": 18, "scoreName": "E-value", "accession": "PF00447.15", "start": 18, "score": 3.3e-33, "identifier": "HSF-type DNA-binding", "type": "DBD", "aliEnd": 118}}, {"startStyle": "straight", "end": 447, "endStyle": "jagged", "aliStart": 247, "text": "Vert_HS_TF", "colour": "#9999ff", "aliEnd": 445, "start": 247, "href": "http://pfam.xfam.org/family/PF06546.9", "type": "pfama", "display": "true", "metadata": {"end": 447, "description": "This family represents the C-terminal region of vertebrate heat shock transcription factors. Heat shock transcription factors regulate the expression of heat shock proteins - a set of proteins that protect the cell from damage caused by stress and aid the cell's recovery after the removal of stress [1]. This C-terminal region is found with the N-terminal Pfam:PF00447, and may contain a three-stranded coiled-coil trimerisation domain and a CE2 regulatory region, the latter of which is  involved in sustained heat shock response [1].", "database": "PfamA", "aliStart": 247, "scoreName": "E-value", "accession": "PF06546.9", "start": 247, "score": 1.4999999999999998e-65, "identifier": "Vertebrate heat shock transcription factor", "type": "DBD", "aliEnd": 445}}, {"startStyle": "jagged", "end": 479, "endStyle": "jagged", "aliStart": 445, "text": "Vert_HS_TF", "colour": "#9999ff", "aliEnd": 471, "start": 444, "href": "http://pfam.xfam.org/family/PF06546.9", "type": "pfama", "display": "true", "metadata": {"end": 479, "description": "This family represents the C-terminal region of vertebrate heat shock transcription factors. Heat shock transcription factors regulate the expression of heat shock proteins - a set of proteins that protect the cell from damage caused by stress and aid the cell's recovery after the removal of stress [1]. This C-terminal region is found with the N-terminal Pfam:PF00447, and may contain a three-stranded coiled-coil trimerisation domain and a CE2 regulatory region, the latter of which is  involved in sustained heat shock response [1].", "database": "PfamA", "aliStart": 445, "scoreName": "E-value", "accession": "PF06546.9", "start": 444, "score": 1.4999999999999998e-65, "identifier": "Vertebrate heat shock transcription factor", "type": "DBD", "aliEnd": 471}}], "length": 496}

{"regions": [{"startStyle": "jagged", "end": 53, "endStyle": "curved", "aliStart": 1, "text": "HSF", "colour": "#009900", "aliEnd": 53, "start": 1, "href": "http://pfam.xfam.org/family/PF00447.15", "type": "pfama", "display": "true", "metadata": {"end": 53, "description": "Heat shock factor (HSF) is a transcriptional activator of heat shock genes [PUBMED:2257625]: it binds specifically to heat shock promoter elements, which are palindromic sequences rich with repetitive purine and pyrimidine motifs [PUBMED:2257625].", "database": "PfamA", "aliStart": 1, "scoreName": "E-value", "accession": "PF00447.15", "start": 1, "score": 3.6000000000000004e-14, "identifier": "HSF-type DNA-binding", "type": "DBD", "aliEnd": 53}}, {"startStyle": "jagged", "end": 128, "endStyle": "straight", "aliStart": 69, "text": "ZapB", "colour": "#9999ff", "aliEnd": 126, "start": 66, "href": "http://pfam.xfam.org/family/PF06005.10", "type": "pfama", "display": "true", "metadata": {"end": 128, "description": "ZapB is a non-essential, abundant cell division factor that is  required for proper Z-ring formation [1]. ", "database": "PfamA", "aliStart": 69, "scoreName": "E-value", "accession": "PF06005.10", "start": 66, "score": 0.0075, "identifier": "Cell division protein ZapB ", "type": "DBD", "aliEnd": 126}}, {"startStyle": "jagged", "end": 138, "endStyle": "jagged", "aliStart": 93, "text": "HMMR_C", "colour": "#9999ff", "aliEnd": 129, "start": 91, "href": "http://pfam.xfam.org/family/PF15908.3", "type": "pfama", "display": "true", "metadata": {"end": 138, "description": "HMMR_C is the C-terminal region of eukaryotic hyaluronan-mediated motility receptor proteins. The protein is functionally associated with BRCA1 and thus predicted to be a common, low-penetrance breast cancer candidate.", "database": "PfamA", "aliStart": 93, "scoreName": "E-value", "accession": "PF15908.3", "start": 91, "score": 0.0093, "identifier": "Hyaluronan mediated motility receptor C-terminal", "type": "DBD", "aliEnd": 129}}], "length": 139}

TF Info Page for HSF1 (HSF)