TF Info Page for WT1 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro Crystal structure (PDB:2JP9) is with dsDNA GCGCAGACGCCCCCGCG, which is consistent with the PWMs

Description

Description: Wilms tumor 1 [Source:HGNC Symbol;Acc:HGNC:12796]
Entrez Summary This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
Ensembl ID: ENSG00000184937
External Link: CisBP
Interpro IPR000976; IPR007087; IPR015880; IPR017987;
Protein Domain: ENSP00000331327
Protein Domain: ENSP00000368370
Protein Domain: ENSP00000415516
Protein Domain: ENSP00000413452
Protein Domain: ENSP00000435307
Domain:
Protein: ENSP00000331327DBD: C2H2 ZF Containing ProteinsOther: WT1
Protein: ENSP00000368370DBD: C2H2 ZF Containing ProteinsOther: WT1
Protein: ENSP00000415516DBD: C2H2 ZF Containing ProteinsOther: WT1
Protein: ENSP00000413452DBD: C2H2 ZF Containing ProteinsOther: WT1
Protein: ENSP00000435307DBD: C2H2 ZF Containing ProteinsOther: WT1

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
ISS - GO_REF:0000024
GO:0003700
sequence-specific DNA binding transcription factor activity
ISS - GO_REF:0000024 | NAS - PMID:7862533, PMID:83
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
PBMBarrera2016Direct
PBMBarrera2016Direct
PBMBarrera2016Direct
PBMBarrera2016Direct
PBMBarrera2016Direct
PBMBarrera2016Direct
PBMBarrera2016Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
TransfacTransfacLicense requiredInferred - Wt1 (98% AA Identity, Mus musculus)

Structure

Structure PDB 2JP9

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution