Crystal structure (PDB:2JP9) is with dsDNA GCGCAGACGCCCCCGCG, which is consistent with the PWMs
Description
Description:
Wilms tumor 1 [Source:HGNC Symbol;Acc:HGNC:12796]
Entrez Summary
This gene encodes a transcription factor that contains four
zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding
domain at the N-terminus. It has an essential role in the normal development
of the urogenital system, and it is mutated in a small subset of patients
with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic
imprinting pattern, with biallelic, and monoallelic expression from the
maternal and paternal alleles in different tissues. Multiple transcript
variants have been described. In several variants, there is evidence for the
use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame
with the first AUG. Authors of PMID:7926762 also provide evidence that WT1
mRNA undergoes RNA editing in human and rat, and that this process is
tissue-restricted and developmentally regulated. [provided by RefSeq, Mar
2015]
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF?
Yes
TFclass considers it as a TF?
Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"
No
GO-Info
GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a ISS - GO_REF:0000024 GO:0003700 sequence-specific DNA binding transcription factor activity ISS - GO_REF:0000024 | NAS - PMID:7862533, PMID:83
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF