The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	POU class 6 homeobox 1 [Source:HGNC Symbol;Acc:HGNC:9224]
Entrez Summary	TBA
Ensembl ID:	ENSG00000184271
External Link:	T093398_1.02
Interpro	IPR000327; IPR001356; IPR009057; IPR010982; IPR013847; ;
Protein Domain:
Protein: ENSP00000330190	DBD: Homeodomain,POU	Other:
Protein: ENSP00000448389	DBD: Homeodomain,POU	Other:
Protein: ENSP00000373895	DBD: Homeodomain,POU	Other:
Protein: ENSP00000490559	DBD: Homeodomain,POU	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding Other_ PMIDS:8645295
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0003700 sequence-specific DNA binding transcription factor activity IEA - GO_REF:0000002
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
PBM	Zoo_01		Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
PBM	Berger08		Inferred - Pou6f1 (99% AA Identity, Mus musculus)
PBM	Berger08		Inferred - Pou6f1 (99% AA Identity, Mus musculus)
PBM	Zoo_01		Inferred - pdm3 (68% AA Identity, Drosophila melanogaster)

Structure

Structure	PDB	3D1N

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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{"regions": [{"startStyle": "curved", "end": 213, "endStyle": "curved", "aliStart": 143, "text": "Pou", "colour": "#2cb42c", "aliEnd": 213, "start": 142, "href": "http://pfam.xfam.org/family/PF00157.15", "type": "pfama", "display": "true", "metadata": {"end": 213, "description": "The POU domain is a bipartite domain composed of two subunits separated by a non-conserved region of 15-55 aa. The N-terminal subunit is known as the POU-specific (POUs) domain (INTERPRO), while the C-terminal subunit is a homeobox domain (INTERPRO). 3D structures of complexes including both POU subdomains bound to DNA are available. Both subdomains contain the structural motif 'helix-turn-helix', which directly associates with the two components of bipartite DNA binding sites, and both are required for high affinity sequence-specific DNA-binding.", "database": "PfamA", "aliStart": 143, "scoreName": "E-value", "accession": "PF00157.15", "start": 142, "score": 1.2e-30, "identifier": "Pou domain - N-terminal to homeobox domain", "type": "DBD", "aliEnd": 213}}, {"startStyle": "curved", "end": 291, "endStyle": "curved", "aliStart": 235, "text": "HD", "colour": "#228B22", "aliEnd": 290, "start": 235, "href": "http://pfam.xfam.org/family/PF00046.27", "type": "pfama", "display": "true", "metadata": {"end": 291, "description": "The homeobox domain or homeodomain was first identified in a number of drosophila homeotic and segmentation proteins, but is now known to be well-conserved in many other animals, including vertebrates [PUBMED:2568852, PUBMED:1357790]. Hox genes encode homeodomain-containing transcriptional regulators that operate differential genetic programs along the anterior-posterior axis of animal bodies [PUBMED:12445403]. The domain binds DNA through a helix-turn-helix (HTH) structure. ", "database": "PfamA", "aliStart": 235, "scoreName": "E-value", "accession": "PF00046.27", "start": 235, "score": 3.9e-19, "identifier": "Homeobox domain", "type": "DBD", "aliEnd": 290}}], "length": 302}

TF Info Page for POU6F1 (Homeodomain; POU)