TF Info Page for GSX2 (Homeodomain)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: GS homeobox 2 [Source:HGNC Symbol;Acc:HGNC:24959]
Entrez Summary NA
Ensembl ID: ENSG00000180613
External Link: CisBP
Interpro IPR001356; IPR009057; IPR017970; IPR020479;
Protein Domain: ENSP00000319118
Protein Domain: ENSP00000483522
Protein Domain: ENSP00000422213
Domain:
Protein: ENSP00000319118DBD: HomeodomainOther:
Protein: ENSP00000483522DBD: HomeodomainOther:
Protein: ENSP00000422213DBD: HomeodomainOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:7619729
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources. 		a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF 		1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
PBMBerger08Inferred - Gsx2 (100% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - GSX1 (98% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - GSX1 (98% AA Identity, Homo sapiens)
B1HJASPARInferred - ind (87% AA Identity, Drosophila melanogaster)
B1HJASPARInferred - zen (75% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - zen (75% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - HOXA4 (70% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA4 (70% AA Identity, Homo sapiens)
PBMBerger08Inferred - Hoxa4 (70% AA Identity, Mus musculus)
B1HJASPARInferred - zen2 (70% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - zen2 (70% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - zen2 (70% AA Identity, Drosophila melanogaster)
PBMZoo_01Inferred - zen2 (70% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - HOXA4 (70% AA Identity, Homo sapiens)
B1HJASPARInferred - Dfd (68% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Dfd (68% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Dfd (68% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - HOXA5 (68% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HOXA5 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA5 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA5 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA5 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA5 (68% AA Identity, Homo sapiens)
PBMBerger08Inferred - Hoxa5 (68% AA Identity, Mus musculus)
PBMBerger08Inferred - Hoxb3 (68% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - HOXB5 (68% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HOXB5 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXB5 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXB5 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXB5 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXB5 (68% AA Identity, Homo sapiens)
PBMBerger08Inferred - Hoxb5 (68% AA Identity, Mus musculus)
PBMBarrera2016Inferred - HOXC4 (68% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HOXC4 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXC4 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXC4 (68% AA Identity, Homo sapiens)
PBMBerger08Inferred - Hoxc4 (68% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - HOXD4 (68% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HOXD4 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXD4 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXD4 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXD4 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXD4 (68% AA Identity, Homo sapiens)
B1HJASPARInferred - pb (68% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - pb (68% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - Dfd (68% AA Identity, Drosophila melanogaster)
COMPILEDJASPARInferred - HOXA5 (68% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HOXA5 (68% AA Identity, Homo sapiens)
MisciDMMPMMInferred - Dfd (68% AA Identity, Drosophila melanogaster)
MiscHocoMocoInferred - HOXA5 (68% AA Identity, Homo sapiens)
MiscHocoMocoInferred - HOXD4 (68% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HOXA2 (66% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HOXA2 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA2 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA2 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA2 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXA2 (66% AA Identity, Homo sapiens)
PBMZoo_01Inferred - HOXA2 (66% AA Identity, Homo sapiens)
PBMBerger08Inferred - Hoxa2 (66% AA Identity, Mus musculus)
PBMBadis09Inferred - Hoxa3 (66% AA Identity, Mus musculus)
PBMBerger08Inferred - Hoxa3 (66% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - HOXB2 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXB2 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXB2 (66% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HOXB4 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXB4 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXB4 (66% AA Identity, Homo sapiens)
PBMBerger08Inferred - Hoxb4 (66% AA Identity, Mus musculus)
PBMBarrera2016Inferred - HOXC4 (66% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HOXD3 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXD3 (66% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HOXD3 (66% AA Identity, Homo sapiens)
PBMBerger08Inferred - Hoxd3 (66% AA Identity, Mus musculus)
PBMZoo_01Inferred - O76842_CUPSA (66% AA Identity, Cupiennius salei)
PBMZoo_01Inferred - SPU_026099 (66% AA Identity, Strongylocentrotus purpuratus)
TransfacTransfacLicense requiredInferred - HOXA3 (66% AA Identity, Homo sapiens)
ChIP-seqmodENCODEInferred - lin-39 (66% AA Identity, Caenorhabditis elegans)
MiscHOMERInferred - Hoxa2 (66% AA Identity, Mus musculus)
MiscHOMERInferred - Hoxb4 (66% AA Identity, Mus musculus)
MiscHOMERInferred - lin-39 (66% AA Identity, Caenorhabditis elegans)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution