The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Likely to be sequence specific TF	1 Monomer or homomultimer	No motif

Description

Description:	zinc fingers and homeoboxes 2 [Source:HGNC Symbol;Acc:HGNC:18513]
Entrez Summary	TBA
Ensembl ID:	ENSG00000178764
External Link:	T093388_1.02
Interpro	IPR001356; IPR007087; IPR009057; IPR015880;
Protein Domain:
Protein: ENSP00000314709	DBD: Homeodomain	Other: DUF3505, Homez
Protein: ENSP00000452720	DBD: Homeodomain	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene Candidate_DNA-Binding sequence-specific_TF PPI Transactivation_ PMIDS:12741956 14659886
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0003700 sequence-specific DNA binding transcription factor activity IDA - PMID:12741956
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	3a, decent circumstantial evidence for its role as
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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", "database": "PfamA", "aliStart": 277, "scoreName": "E-value", "accession": "PF00046.27", "start": 272, "score": 1.4000000000000002e-25, "identifier": "Homeobox domain", "type": "DBD", "aliEnd": 317}}, {"startStyle": "jagged", "end": 496, "endStyle": "curved", "aliStart": 446, "text": "HD", "colour": "#228B22", "aliEnd": 495, "start": 442, "href": "http://pfam.xfam.org/family/PF00046.27", "type": "pfama", "display": "true", "metadata": {"end": 496, "description": "The homeobox domain or homeodomain was first identified in a number of drosophila homeotic and segmentation proteins, but is now known to be well-conserved in many other animals, including vertebrates [PUBMED:2568852, PUBMED:1357790]. Hox genes encode homeodomain-containing transcriptional regulators that operate differential genetic programs along the anterior-posterior axis of animal bodies [PUBMED:12445403]. The domain binds DNA through a helix-turn-helix (HTH) structure. ", "database": "PfamA", "aliStart": 446, "scoreName": "E-value", "accession": "PF00046.27", "start": 442, "score": 1.4000000000000002e-25, "identifier": "Homeobox domain", "type": "DBD", "aliEnd": 495}}, {"startStyle": "jagged", "end": 584, "endStyle": "curved", "aliStart": 537, "text": "HD", "colour": "#228B22", "aliEnd": 581, "start": 534, "href": "http://pfam.xfam.org/family/PF00046.27", "type": "pfama", "display": "true", "metadata": {"end": 584, "description": "The homeobox domain or homeodomain was first identified in a number of drosophila homeotic and segmentation proteins, but is now known to be well-conserved in many other animals, including vertebrates [PUBMED:2568852, PUBMED:1357790]. Hox genes encode homeodomain-containing transcriptional regulators that operate differential genetic programs along the anterior-posterior axis of animal bodies [PUBMED:12445403]. The domain binds DNA through a helix-turn-helix (HTH) structure. ", "database": "PfamA", "aliStart": 537, "scoreName": "E-value", "accession": "PF00046.27", "start": 534, "score": 1.4000000000000002e-25, "identifier": "Homeobox domain", "type": "DBD", "aliEnd": 581}}, {"startStyle": "jagged", "end": 685, "endStyle": "curved", "aliStart": 636, "text": "HD", "colour": "#228B22", "aliEnd": 681, "start": 633, "href": "http://pfam.xfam.org/family/PF00046.27", "type": "pfama", "display": "true", "metadata": {"end": 685, "description": "The homeobox domain or homeodomain was first identified in a number of drosophila homeotic and segmentation proteins, but is now known to be well-conserved in many other animals, including vertebrates [PUBMED:2568852, PUBMED:1357790]. Hox genes encode homeodomain-containing transcriptional regulators that operate differential genetic programs along the anterior-posterior axis of animal bodies [PUBMED:12445403]. The domain binds DNA through a helix-turn-helix (HTH) structure. ", "database": "PfamA", "aliStart": 636, "scoreName": "E-value", "accession": "PF00046.27", "start": 633, "score": 1.4000000000000002e-25, "identifier": "Homeobox domain", "type": "DBD", "aliEnd": 681}}, {"startStyle": "jagged", "end": 769, "endStyle": "straight", "aliStart": 729, "text": "Homez", "colour": "#9999ff", "aliEnd": 766, "start": 717, "href": "http://pfam.xfam.org/family/PF11569.6", "type": "pfama", "display": "true", "metadata": {"end": 769, "description": "Homez contains two leucine zipper-like motifs and an acidic domain and belongs to the superfamily of homeobox-containing proteins. The presence of leucine zippers suggests that Homez can function as a homo or heterodimer in the nucleus [1]. It is thought that the first leucine zipper and homeodomain 1 (HD1)of Homez is responsible for dimerisation and HD2 has a specific DNA-binding activity. Homez is also thought to function as a transcriptional repressor due to the acidic region in its C-terminal domain [1]. Homez is involved in a complex regulatory network [1].", "database": "PfamA", "aliStart": 729, "scoreName": "E-value", "accession": "PF11569.6", "start": 717, "score": 7.4e-07, "identifier": "Homeodomain leucine-zipper encoding, Homez", "type": "DBD", "aliEnd": 766}}, {"startStyle": "jagged", "end": 102, "endStyle": "straight", "aliStart": 74, "text": "DUF3505", "colour": "#9999ff", "aliEnd": 101, "start": 41, "href": "http://pfam.xfam.org/family/PF12013.6", "type": "pfama", "display": "true", "metadata": {"end": 102, "description": "This family of proteins is functionally uncharacterised. This protein is found in eukaryotes. Proteins in this family are typically between 247 to 1018 amino acids in length. This region contains two segments that are likely to be C2H2 zinc binding domains.", "database": "PfamA", "aliStart": 74, "scoreName": "E-value", "accession": "PF12013.6", "start": 41, "score": 0.0008, "identifier": "Protein of unknown function (DUF3505)", "type": "DBD", "aliEnd": 101}}, {"startStyle": "jagged", "end": 134, "endStyle": "straight", "aliStart": 110, "text": "DUF3505", "colour": "#9999ff", "aliEnd": 133, "start": 105, "href": "http://pfam.xfam.org/family/PF12013.6", "type": "pfama", "display": "true", "metadata": {"end": 134, "description": "This family of proteins is functionally uncharacterised. This protein is found in eukaryotes. Proteins in this family are typically between 247 to 1018 amino acids in length. This region contains two segments that are likely to be C2H2 zinc binding domains.", "database": "PfamA", "aliStart": 110, "scoreName": "E-value", "accession": "PF12013.6", "start": 105, "score": 0.0008, "identifier": "Protein of unknown function (DUF3505)", "type": "DBD", "aliEnd": 133}}], "length": 838}

{"regions": [{"startStyle": "curved", "end": 101, "endStyle": "curved", "aliStart": 78, "text": "zfC2H2", "colour": "#228B22", "aliEnd": 101, "start": 78, "href": "http://pfam.xfam.org/family/PF00096.24", "type": "pfama", "display": "true", "metadata": {"end": 101, "description": "The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity  site for Sp1 binding to the wt1 promoter [2].", "database": "PfamA", "aliStart": 78, "scoreName": "E-value", "accession": "PF00096.24", "start": 78, "score": 0.00091, "identifier": "Zinc finger, C2H2 type", "type": "DBD", "aliEnd": 101}}, {"startStyle": "curved", "end": 133, "endStyle": "curved", "aliStart": 110, "text": "zfC2H2", "colour": "#228B22", "aliEnd": 133, "start": 110, "href": "http://pfam.xfam.org/family/PF00096.24", "type": "pfama", "display": "true", "metadata": {"end": 133, "description": "The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity  site for Sp1 binding to the wt1 promoter [2].", "database": "PfamA", "aliStart": 110, "scoreName": "E-value", "accession": "PF00096.24", "start": 110, "score": 0.00091, "identifier": "Zinc finger, C2H2 type", "type": "DBD", "aliEnd": 133}}], "length": 166}

TF Info Page for ZHX2 (Homeodomain)