The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	MAF bZIP transcription factor [Source:HGNC Symbol;Acc:HGNC:6776]
Entrez Summary	TBA
Ensembl ID:	ENSG00000178573
External Link:	T025317_1.02
Interpro	IPR004826; IPR004827; IPR008917; IPR013592;
Protein Domain:
Protein: ENSP00000327048	DBD: bZIP	Other: Maf_N
Protein: ENSP00000455097	DBD: bZIP	Other: Maf_N
Protein: ENSP00000377019	DBD: bZIP	Other: Maf_N

Previous Annotations

Source	Annotation
TF-CAT classification	No PMIDS:
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001228 RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor IBA - GO_REF:0000033
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
Transfac	Transfac	License required	Inferred - MAF_AVIS4 (98% AA Identity, Avian musculoaponeurotic fibrosarcoma virus AS42)
Transfac	Transfac	License required	Inferred - MAF_CHICK (98% AA Identity, Gallus gallus)
HT-SELEX	Yin2017		Inferred - MAFA (79% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - MAFA (79% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - MAFA (79% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - MAFA (79% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - MAFA (79% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - MAFA (79% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - MAFA (79% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - MAFA (79% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - MAFA (79% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - MAFA (79% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - MAFA (79% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - Mafa (79% AA Identity, Mus musculus)
PBM	Badis09		Inferred - Mafb (77% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - MAFB (77% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - MAFB (77% AA Identity, Homo sapiens)
SELEX	JASPAR		Inferred - Mafb (77% AA Identity, Mus musculus)
Misc	HocoMoco		Inferred - MAFB (77% AA Identity, Homo sapiens)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 352, "endStyle": "curved", "aliStart": 261, "text": "MAF", "colour": "#FFD800", "aliEnd": 351, "start": 261, "href": "http://pfam.xfam.org/family/PF03131.15", "type": "pfama", "display": "true", "metadata": {"end": 352, "description": "Maf transcription factors contain a conserved basic region leucine zipper (bZIP) domain, which mediates their dimerisation and DNA binding property [1]. Thus, this family is probably related to Pfam:PF00170. This family also includes the DNA_binding domain of Skn-1 (Swiss:P34707), this domain lacks the leucine zipper found in other bZip domains, and binds DNA is a monomer [2,3].", "database": "PfamA", "aliStart": 261, "scoreName": "E-value", "accession": "PF03131.15", "start": 261, "score": 1e-34, "identifier": "bZIP Maf transcription factor", "type": "DBD", "aliEnd": 351}}, {"startStyle": "curved", "end": 353, "endStyle": "curved", "aliStart": 288, "text": "bZIP", "colour": "#009900", "aliEnd": 347, "start": 287, "href": "http://pfam.xfam.org/family/PF00170.19", "type": "pfama", "display": "true", "metadata": {"end": 353, "description": "The Pfam entry includes the basic region and the leucine zipper region.", "database": "PfamA", "aliStart": 288, "scoreName": "E-value", "accession": "PF00170.19", "start": 287, "score": 0.0081, "identifier": "bZIP transcription factor", "type": "DBD", "aliEnd": 347}}, {"startStyle": "straight", "end": 119, "endStyle": "straight", "aliStart": 87, "text": "Maf_N", "colour": "#9999ff", "aliEnd": 119, "start": 86, "href": "http://pfam.xfam.org/family/PF08383.9", "type": "pfama", "display": "true", "metadata": {"end": 119, "description": "This region is found in various leucine zipper transcription factors of the Maf family. These are implicated in the regulation of insulin gene expression [1], in erythroid differentiation [2], and in differentiation of the neuroretina [3].", "database": "PfamA", "aliStart": 87, "scoreName": "E-value", "accession": "PF08383.9", "start": 86, "score": 2.2000000000000002e-20, "identifier": "Maf N-terminal region", "type": "DBD", "aliEnd": 119}}], "length": 404}

{"regions": [{"startStyle": "curved", "end": 352, "endStyle": "curved", "aliStart": 261, "text": "MAF", "colour": "#FFD800", "aliEnd": 351, "start": 261, "href": "http://pfam.xfam.org/family/PF03131.15", "type": "pfama", "display": "true", "metadata": {"end": 352, "description": "Maf transcription factors contain a conserved basic region leucine zipper (bZIP) domain, which mediates their dimerisation and DNA binding property [1]. Thus, this family is probably related to Pfam:PF00170. This family also includes the DNA_binding domain of Skn-1 (Swiss:P34707), this domain lacks the leucine zipper found in other bZip domains, and binds DNA is a monomer [2,3].", "database": "PfamA", "aliStart": 261, "scoreName": "E-value", "accession": "PF03131.15", "start": 261, "score": 9.499999999999999e-35, "identifier": "bZIP Maf transcription factor", "type": "DBD", "aliEnd": 351}}, {"startStyle": "curved", "end": 353, "endStyle": "curved", "aliStart": 288, "text": "bZIP", "colour": "#009900", "aliEnd": 347, "start": 287, "href": "http://pfam.xfam.org/family/PF00170.19", "type": "pfama", "display": "true", "metadata": {"end": 353, "description": "The Pfam entry includes the basic region and the leucine zipper region.", "database": "PfamA", "aliStart": 288, "scoreName": "E-value", "accession": "PF00170.19", "start": 287, "score": 0.0074, "identifier": "bZIP transcription factor", "type": "DBD", "aliEnd": 347}}, {"startStyle": "straight", "end": 119, "endStyle": "straight", "aliStart": 87, "text": "Maf_N", "colour": "#9999ff", "aliEnd": 119, "start": 86, "href": "http://pfam.xfam.org/family/PF08383.9", "type": "pfama", "display": "true", "metadata": {"end": 119, "description": "This region is found in various leucine zipper transcription factors of the Maf family. These are implicated in the regulation of insulin gene expression [1], in erythroid differentiation [2], and in differentiation of the neuroretina [3].", "database": "PfamA", "aliStart": 87, "scoreName": "E-value", "accession": "PF08383.9", "start": 86, "score": 2.1000000000000002e-20, "identifier": "Maf N-terminal region", "type": "DBD", "aliEnd": 119}}], "length": 384}

{"regions": [{"startStyle": "curved", "end": 352, "endStyle": "curved", "aliStart": 261, "text": "MAF", "colour": "#FFD800", "aliEnd": 351, "start": 261, "href": "http://pfam.xfam.org/family/PF03131.15", "type": "pfama", "display": "true", "metadata": {"end": 352, "description": "Maf transcription factors contain a conserved basic region leucine zipper (bZIP) domain, which mediates their dimerisation and DNA binding property [1]. Thus, this family is probably related to Pfam:PF00170. This family also includes the DNA_binding domain of Skn-1 (Swiss:P34707), this domain lacks the leucine zipper found in other bZip domains, and binds DNA is a monomer [2,3].", "database": "PfamA", "aliStart": 261, "scoreName": "E-value", "accession": "PF03131.15", "start": 261, "score": 9.1e-35, "identifier": "bZIP Maf transcription factor", "type": "DBD", "aliEnd": 351}}, {"startStyle": "curved", "end": 353, "endStyle": "curved", "aliStart": 288, "text": "bZIP", "colour": "#009900", "aliEnd": 347, "start": 287, "href": "http://pfam.xfam.org/family/PF00170.19", "type": "pfama", "display": "true", "metadata": {"end": 353, "description": "The Pfam entry includes the basic region and the leucine zipper region.", "database": "PfamA", "aliStart": 288, "scoreName": "E-value", "accession": "PF00170.19", "start": 287, "score": 0.0071, "identifier": "bZIP transcription factor", "type": "DBD", "aliEnd": 347}}, {"startStyle": "straight", "end": 119, "endStyle": "straight", "aliStart": 87, "text": "Maf_N", "colour": "#9999ff", "aliEnd": 119, "start": 86, "href": "http://pfam.xfam.org/family/PF08383.9", "type": "pfama", "display": "true", "metadata": {"end": 119, "description": "This region is found in various leucine zipper transcription factors of the Maf family. These are implicated in the regulation of insulin gene expression [1], in erythroid differentiation [2], and in differentiation of the neuroretina [3].", "database": "PfamA", "aliStart": 87, "scoreName": "E-value", "accession": "PF08383.9", "start": 86, "score": 2.0000000000000002e-20, "identifier": "Maf N-terminal region", "type": "DBD", "aliEnd": 119}}], "length": 374}

TF Info Page for MAF (bZIP)