TF Info Page for JUN (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Jun proto-oncogene, AP-1 transcription factor subunit [Source:HGNC Symbol;Acc:HGNC:6204]
Entrez Summary This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000177606
External Link: CisBP
Interpro IPR002112; IPR004827; IPR005643; IPR008917;
Protein Domain: ENSP00000360266
Domain:
Protein: ENSP00000360266DBD: bZIPOther: Jun

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:12215258 15494506
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IC - PMID:21113145
GO:0000982
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IMP - PMID:19962668
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:9732876
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
IC - PMID:19861239 | IDA - PMID:2833704
GO:0051090
regulation of sequence-specific DNA binding transcription factor activity
TAS - Reactome:R-HSA-450341
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
SMiLE-seqIsakova2017Direct
SMiLE-seqIsakova2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqJASPARDirect
ChIP-seqJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMMann2013Inferred - Jun (100% AA Identity, Mus musculus)
PBMZoo_01Inferred - Jun (100% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - JUN_AVIS1 (98% AA Identity, Avian sarcoma virus 17)
PBMZoo_01Inferred - jund (90% AA Identity, Xenopus tropicalis)
SMiLE-seqIsakova2017Inferred - JUND (89% AA Identity, Homo sapiens)
SMiLE-seqIsakova2017Inferred - JUND (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - JUND (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - JUND (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - JUND (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - JUND (89% AA Identity, Homo sapiens)
PBMMann2013Inferred - Jund (89% AA Identity, Mus musculus)
PBMMann2013Inferred - Jund (89% AA Identity, Mus musculus)
PBMZoo_01Inferred - Jund (89% AA Identity, Mus musculus)
ChIP-seqENCODEInferred - JUND (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - JUND (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - JUND (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - JUND (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - JUND (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - JUND (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - JUND (89% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - JUND (89% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - JUND (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - JUND (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - JUND (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - JUND (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - JUND (89% AA Identity, Homo sapiens)
MiscHocoMocoInferred - JUND (89% AA Identity, Homo sapiens)
MiscHOMERInferred - JUND (89% AA Identity, Homo sapiens)
SMiLE-seqIsakova2017Inferred - JUNB (82% AA Identity, Homo sapiens)
SMiLE-seqIsakova2017Inferred - JUNB (82% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - JUNB (82% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - JUNB (82% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - JUNB (82% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - JUNB (82% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - JUNB (82% AA Identity, Homo sapiens)
MiscHocoMocoInferred - JUNB (82% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Junb (81% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - Jra (67% AA Identity, Drosophila melanogaster)

Structure

Structure PDB 1JNM

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution