TF Info Page for IRX5 (Homeodomain)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: iroquois homeobox 5 [Source:HGNC Symbol;Acc:HGNC:14361]
Entrez Summary NA
Ensembl ID: ENSG00000176842
External Link: CisBP
Interpro IPR001356; IPR003893; IPR008422; IPR009057; IPR017970; ;
Protein Domain: ENSP00000316250
Protein Domain: ENSP00000483446
Protein Domain: ENSP00000378132
Protein Domain: ENSP00000453660
Domain:
Protein: ENSP00000316250DBD: HomeodomainOther:
Protein: ENSP00000483446DBD: HomeodomainOther:
Protein: ENSP00000378132DBD: HomeodomainOther:
Protein: ENSP00000453660DBD: HomeodomainOther:

Previous Annotations

Source Annotation
TF-CAT classification Indeterminate - There is no evidence for or against this genes role as a TF___
PMIDS:0
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
b
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
PBMBerger08Inferred - Irx2 (100% AA Identity, Mus musculus)
PBMBerger08Inferred - Irx5 (100% AA Identity, Mus musculus)
B1HJASPARInferred - ara (91% AA Identity, Drosophila melanogaster)
B1HJASPARInferred - caup (90% AA Identity, Drosophila melanogaster)
B1HJASPARInferred - mirr (90% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - IRX1 (88% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - IRX1 (88% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - IRX3 (88% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - IRX3 (88% AA Identity, Homo sapiens)
PBMBerger08Inferred - Irx3 (88% AA Identity, Mus musculus)
PBMBerger08Inferred - Irx3 (88% AA Identity, Mus musculus)
PBMBerger08Inferred - Irx4 (88% AA Identity, Mus musculus)
PBMBerger08Inferred - Irx6 (86% AA Identity, Mus musculus)
PBMZoo_01Inferred - SNAPOd2T00005194001 (75% AA Identity, Oikopleura dioica)
PBMZoo_01Inferred - Cbr-irx-1 (71% AA Identity, Caenorhabditis briggsae)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution