TF Info Page for DDIT3 (bZIP)


Assessment Binding Mode Motif Status Notes Comments
Known motif 2 Obligate heteromer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Has strong preference for forming heterodimers with BATF; DBP; HLF; CEBP[ABCD] over homodimerization (PMID: 12805554).


Description: DNA damage inducible transcript 3 [Source:HGNC Symbol;Acc:HGNC:2726]
Entrez Summary This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
Ensembl ID: ENSG00000175197
External Link: CisBP
Interpro IPR004827; IPR016670;
Protein Domain: ENSP00000340671
Protein Domain: ENSP00000448665
Protein Domain: ENSP00000447803
Protein Domain: ENSP00000447188
Protein Domain: ENSP00000447503
Protein: ENSP00000340671DBD: bZIPOther:
Protein: ENSP00000448665DBD: bZIPOther:
Protein: ENSP00000447803DBD: bZIPOther:
Protein: ENSP00000447188DBD: bZIPOther:
Protein: ENSP00000447503DBD: bZIPOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
Transcription Factor Binding
tf co-factor binding_Co-activation_
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:22065586
sequence-specific DNA binding transcription factor activity
IC - PMID:15322075 | IGI - PMID:24939851
negative regulation of CREB transcription factor activity
IDA - PMID:8622660 | IGI - PMID:11478948
negative regulation of sequence-specific DNA binding transcription factor activity
IDA - PMID:16434966
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements Obligate_Multimer


Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
MiscHOMERInferred - Ddit3 (92% AA Identity, Mus musculus)


Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution