TF Info Page for RELA (Rel)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source Can bind as a homodimer or heterodimer (PMID: 22101729)

Description

Description: RELA proto-oncogene, NF-kB subunit [Source:HGNC Symbol;Acc:HGNC:9955]
Entrez Summary NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Ensembl ID: ENSG00000173039
External Link: CisBP
Interpro IPR000451; IPR002909; IPR008967; IPR011539; IPR014756; IPR030492;
Protein Domain: ENSP00000311508
Protein Domain: ENSP00000384273
Protein Domain: ENSP00000432537
Protein Domain: ENSP00000431304
Protein Domain: ENSP00000483705
Protein Domain: ENSP00000434098
Domain:
Protein: ENSP00000311508DBD: RHDOther: RHD_dimer
Protein: ENSP00000384273DBD: RHDOther: RHD_dimer
Protein: ENSP00000432537DBD: RHDOther: RHD_dimer
Protein: ENSP00000431304DBD: RHDOther:
Protein: ENSP00000483705DBD: RHDOther: RHD_dimer
Protein: ENSP00000434098DBD: RHDOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:1935902 15485852 15857826
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000983
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity
IMP - PMID:26268439
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:17350185
GO:0001078
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:17350185
GO:0001205
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involv
IDA - PMID:1406630
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:12048232
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
IDA - PMID:3091258
GO:0051092
positive regulation of NF-kappaB transcription factor activity
IDA - PMID:15790681 | TAS - Reactome:R-HSA-445989
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
SELEXJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERDirect
ChIP-seqhmCHIPInferred - Rela (93% AA Identity, Mus musculus)
ChIP-seqhmCHIPInferred - Rela (93% AA Identity, Mus musculus)

Structure

Structure PDB 3GUT

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution