TF Info Page for SP3 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Sp3 transcription factor [Source:HGNC Symbol;Acc:HGNC:11208]
Entrez Summary This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]
Ensembl ID: ENSG00000172845
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000310301
Protein Domain: ENSP00000413665
Protein Domain: ENSP00000406140
Domain:
Protein: ENSP00000310301DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000413665DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000406140DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:12591615
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
GO:0001078
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
ChIP-seqSchmitges2016Inferred - SP4 (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP4 (91% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP4 (91% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Sp1 (90% AA Identity, Mus musculus)
PBMBadis09Inferred - Sp4 (90% AA Identity, Mus musculus)
SMiLE-seqIsakova2017Inferred - Sp4 (90% AA Identity, Mus musculus)
PBMZoo_01Inferred - Sp4 (90% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - SP1 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP1 (89% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - SP1 (89% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - SP1 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (89% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP1 (89% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP1 (89% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - Sp1 (87% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - SP9 (87% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP9 (87% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - SP8 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP8 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP5 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP7 (80% AA Identity, Homo sapiens)
PBMLam11Inferred - ZFA5_CONSTRUCT (78% AA Identity, PBM CONSTRUCTS)
HT-SELEXYin2017Inferred - SP2 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP2 (76% AA Identity, Homo sapiens)
PBMLam11Inferred - ZFA93_CONSTRUCT (76% AA Identity, PBM CONSTRUCTS)
ChIP-seqENCODEInferred - SP2 (76% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP2 (76% AA Identity, Homo sapiens)
PBMLam11Inferred - ZFA4_CONSTRUCT (73% AA Identity, PBM CONSTRUCTS)
PBMCEPDInferred - sptf-3 (72% AA Identity, Caenorhabditis elegans)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution