TF Info Page for NHLH1 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: nescient helix-loop-helix 1 [Source:HGNC Symbol;Acc:HGNC:7817]
Entrez Summary The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).[supplied by OMIM, Nov 2002]
Ensembl ID: ENSG00000171786
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000302189
Domain:
Protein: ENSP00000302189DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
b
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IDA - PMID:12878195
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
SELEXJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
HT-SELEXYin2017Inferred - NHLH2 (98% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NHLH2 (98% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Nhlh2 (98% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - Nhlh2 (98% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - HLH4C (92% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - HLH4C (92% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - HLH4C (92% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - TAL1 (62% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TAL1 (62% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TAL1 (62% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TAL1 (62% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TAL1 (62% AA Identity, Homo sapiens)
MiscHOMERInferred - Tal1 (62% AA Identity, Mus musculus)
MiscHOMERInferred - Tal1 (62% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - HAND2 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HAND2 (50% AA Identity, Homo sapiens)
MiscHocoMocoInferred - HAND1 (50% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution