TF Info Page for BPTF (Unknown)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain; Has a putative AT-hook (PMID: 10575013) obtained a motif by SELEX and showed that mutating the sequence diminished binding in vitro. Unusual for a PHD/Ring finger, but its there in the literature.

Description

Description: bromodomain PHD finger transcription factor [Source:HGNC Symbol;Acc:HGNC:3581]
Entrez Summary This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif, suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought, and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila, the NURF complex, which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors, is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000171634
External Link: CisBP
Interpro IPR001487; IPR001965; IPR004022; IPR011011; IPR018359; IPR018500; IPR018501; IPR019786; IPR019787; IPR028942;
Protein Domain: ENSP00000307208
Protein Domain: ENSP00000343837
Protein Domain: ENSP00000388405
Protein Domain: ENSP00000462535
Protein Domain: ENSP00000463845
Protein Domain: ENSP00000463736
Protein Domain: ENSP00000443949
Protein Domain: ENSP00000462703
Domain:
Protein: ENSP00000307208DBD: OtherOther: Bromodomain, DDT, PHD, WHIM1, WSD
Protein: ENSP00000343837DBD: OtherOther: Bromodomain, PHD
Protein: ENSP00000388405DBD: OtherOther: Bromodomain, DDT, PHD, WHIM1, WSD
Protein: ENSP00000462535DBD: OtherOther:
Protein: ENSP00000463845DBD: OtherOther: Bromodomain, DUF4428, PHD
Protein: ENSP00000463736DBD: OtherOther: Bromodomain
Protein: ENSP00000443949DBD: OtherOther:
Protein: ENSP00000462703DBD: OtherOther: Bromodomain

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding
DNA-Binding
sequence-specific_DNA Binding_
PMIDS:10575013 10727212
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
No
CisBP considers it as a TF? Yes
TFclass considers it as a TF? No
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution