TF Info Page for NEUROD2 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: neuronal differentiation 2 [Source:HGNC Symbol;Acc:HGNC:7763]
Entrez Summary This gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000171532
External Link: CisBP
Interpro IPR011598; IPR016637; IPR022575; ;
Protein Domain: ENSP00000306754
Domain:
Protein: ENSP00000306754DBD: bHLHOther: Neuro_bHLH

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:8816493 10098882 10871374
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:8816493
GO:0051091
positive regulation of sequence-specific DNA binding transcription factor activity
ISS - GO_REF:0000024
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
HT-SELEXYin2017Inferred - NEUROD1 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NEUROD1 (96% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NEUROD1 (96% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NEUROD1 (96% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NEUROD1 (96% AA Identity, Homo sapiens)
MiscHOMERInferred - Neurod1 (96% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - NEUROG1 (68% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NEUROG1 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NEUROG1 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NEUROG1 (68% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Neurog1 (68% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - NEUROG2 (68% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NEUROG2 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NEUROG2 (68% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NEUROG2 (68% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ATOH1 (59% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ATOH1 (59% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ATOH1 (59% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ATOH1 (59% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Atoh1 (59% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ATOH1 (59% AA Identity, Homo sapiens)
SELEXJASPARInferred - Atoh1 (59% AA Identity, Mus musculus)
MiscHOMERInferred - Atoh1 (59% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - BHLHA15 (58% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - BHLHA15 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHA15 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHA15 (58% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Bhlha15 (58% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - ATOH7 (57% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ATOH7 (57% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - dimm (56% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - dimm (56% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - BHLHE22 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHE22 (55% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Bhlhe22 (55% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - BHLHE23 (55% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - BHLHE23 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHE23 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHE23 (55% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - Oli (55% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - amos (53% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - amos (53% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - OLIG2 (53% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - OLIG2 (53% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - OLIG2 (53% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - OLIG2 (53% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - OLIG3 (53% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - OLIG3 (53% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - OLIG3 (53% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - OLIG3 (53% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - OLIG3 (53% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - OLIG3 (53% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Olig3 (53% AA Identity, Mus musculus)
MiscHOMERInferred - Olig2 (53% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - FERD3L (50% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - FERD3L (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - FERD3L (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - FERD3L (50% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution