The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description:

forkhead box N2 [Source:HGNC Symbol;Acc:HGNC:5281]

Entrez Summary

Ensembl ID:

ENSG00000170802

External Link:

CisBP

Interpro

IPR001766; IPR018122;

Protein Domain:

ENSP00000343633

Protein Domain:

ENSP00000388486

Protein Domain:

ENSP00000484534

Domain:

Source	Annotation
TF-CAT classification	No PMIDS:
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0003700 sequence-specific DNA binding transcription factor activity IEA - GO_REF:0000002
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

Source	Annotation	Motif	Evidence
PBM	Nakagawa2013		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
PBM	TF_Evolution	Unpublished Data	Inferred - FOXN3 (74% AA Identity, Homo sapiens)
PBM	Nakagawa2013		Inferred - TriadG17577 (73% AA Identity, Trichoplax adhaerens)
PBM	Nakagawa2013		Inferred - NEMVEDRAFT_v1g67209 (72% AA Identity, Nematostella vectensis)
PBM	Nakagawa2013		Inferred - CHES-1-like (70% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - CHES-1-like (70% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - CHES-1-like (70% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - CHES-1-like (70% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - CHES-1-like (70% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - CHES-1-like (70% AA Identity, Drosophila melanogaster)

Structure	PDB	Not_Covered

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)