TF Info Page for EMX2 (Homeodomain)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: empty spiracles homeobox 2 [Source:HGNC Symbol;Acc:HGNC:3341]
Entrez Summary This gene encodes a homeobox-containing transcription factor that is the homolog to the 'empty spiracles' gene in Drosophila. Research on this gene in humans has focused on its expression in three tissues: dorsal telencephalon, olfactory neuroepithelium, and urogenetial system. It is expressed in the dorsal telencephalon during development in a low rostral-lateral to high caudal-medial gradient and is proposed to pattern the neocortex into defined functional areas. It is also expressed in embryonic and adult olfactory neuroepithelia where it complexes with eukaryotic translation initiation factor 4E (eIF4E) and possibly regulates mRNA transport or translation. In the developing urogenital system, it is expressed in epithelial tissues and is negatively regulated by HOXA10. Alternative splicing results in multiple transcript variants encoding distinct proteins.[provided by RefSeq, Sep 2009]
Ensembl ID: ENSG00000170370
External Link: CisBP
Interpro IPR000047; IPR001356; IPR009057; IPR017970; IPR020479; ;
Protein Domain: ENSP00000450962
Protein Domain: ENSP00000474874
Protein Domain: ENSP00000480271
Domain:
Protein: ENSP00000450962DBD: HomeodomainOther:
Protein: ENSP00000474874DBD: HomeodomainOther:
Protein: ENSP00000480271DBD: HomeodomainOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene Candidate_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:1352754 16919471
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
PBMBerger08Inferred - Emx2 (100% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - EMX1 (92% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - EMX1 (92% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - EMX1 (92% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - EMX1 (92% AA Identity, Homo sapiens)
PBMZoo_01Inferred - EMX1 (92% AA Identity, Homo sapiens)
B1HJASPARInferred - E5 (87% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - E5 (87% AA Identity, Drosophila melanogaster)
B1HJASPARInferred - ems (84% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - ems (84% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - ems (84% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - ems (84% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - ems (84% AA Identity, Drosophila melanogaster)
PBMZoo_01Inferred - ems (84% AA Identity, Drosophila melanogaster)
MisciDMMPMMInferred - ems (84% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution