TF Info Page for SMAD1 (SMAD)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 2 Obligate heteromer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Well-established obligate heteromer

Description

Description: SMAD family member 1 [Source:HGNC Symbol;Acc:HGNC:6767]
Entrez Summary The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000170365
External Link: CisBP
Interpro IPR001132; IPR003619; IPR008984; IPR013019;
Protein Domain: ENSP00000305769
Protein Domain: ENSP00000377652
Protein Domain: ENSP00000427002
Protein Domain: ENSP00000425270
Protein Domain: ENSP00000426568
Protein Domain: ENSP00000421601
Domain:
Protein: ENSP00000305769DBD: MH1 Family DBDsOther: IRF-3, MH2
Protein: ENSP00000377652DBD: MH1 Family DBDsOther: IRF-3, MH2
Protein: ENSP00000427002DBD: MH1 Family DBDsOther:
Protein: ENSP00000425270DBD: MH1 Family DBDsOther:
Protein: ENSP00000426568DBD: MH1 Family DBDsOther: IRF-3, MH2
Protein: ENSP00000421601DBD: MH1 Family DBDsOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:12270938
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:9389648
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
TransfacTransfacLicense requiredInferred - SMAD9 (95% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Smad9 (95% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - Smad9 (95% AA Identity, Rattus norvegicus)
ChIP-chipJASPARInferred - Mad (88% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - Mad (88% AA Identity, Drosophila melanogaster)
MisciDMMPMMInferred - Mad (88% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - Smad5 (74% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - Smad5 (74% AA Identity, Rattus norvegicus)
HT-SELEXYin2017Inferred - SMAD5 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SMAD5 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SMAD5 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SMAD5 (72% AA Identity, Homo sapiens)

Structure

Structure PDB 3KMP

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution