The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	Fos proto-oncogene, AP-1 transcription factor subunit [Source:HGNC Symbol;Acc:HGNC:3796]
Entrez Summary	TBA
Ensembl ID:	ENSG00000170345
External Link:
Interpro	IPR000837; IPR004827;
Protein Domain:
Protein: ENSP00000306245	DBD: bZIP	Other:
Protein: ENSP00000442268	DBD: bZIP	Other:
Protein: ENSP00000450519	DBD: bZIP	Other:
Protein: ENSP00000452386	DBD: bZIP	Other:
Protein: ENSP00000450886	DBD: bZIP	Other:
Protein: ENSP00000452440	DBD: bZIP	Other: DUF3450, IFT20
Protein: ENSP00000452590	DBD: bZIP	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:8798512 11925568 15073183 2243767
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0000982 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IBA - GO_REF:0000033 GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IEA - GO_REF:0000019 GO:0003700 sequence-specific DNA binding transcription factor activity IDA - PMID:9732876 GO:0051090 regulation of sequence-specific DNA binding transcription factor activity TAS - Reactome:R-HSA-450341
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	JASPAR		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
HT-SELEX	Yin2017		Inferred - FOSB (79% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FOSB (79% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - FOSB (79% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
PBM	Zoo_01		Inferred - Fosl1 (75% AA Identity, Mus musculus)
ChIP-seq	ENCODE		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
ChIP-seq	JASPAR		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - FOSL1 (75% AA Identity, Homo sapiens)
PBM	Zoo_01		Inferred - Fosl2 (58% AA Identity, Mus musculus)
Misc	HOMER		Inferred - Fosl2 (58% AA Identity, Mus musculus)

Structure

Structure	PDB	1A02

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 197, "endStyle": "curved", "aliStart": 135, "text": "bZIP", "colour": "#009900", "aliEnd": 194, "start": 135, "href": "http://pfam.xfam.org/family/PF00170.19", "type": "pfama", "display": "true", "metadata": {"end": 197, "description": "The Pfam entry includes the basic region and the leucine zipper region.", "database": "PfamA", "aliStart": 135, "scoreName": "E-value", "accession": "PF00170.19", "start": 135, "score": 6.7000000000000004e-09, "identifier": "bZIP transcription factor", "type": "DBD", "aliEnd": 194}}, {"startStyle": "jagged", "end": 200, "endStyle": "curved", "aliStart": 139, "text": "MAF", "colour": "#FFD800", "aliEnd": 194, "start": 125, "href": "http://pfam.xfam.org/family/PF03131.15", "type": "pfama", "display": "true", "metadata": {"end": 200, "description": "Maf transcription factors contain a conserved basic region leucine zipper (bZIP) domain, which mediates their dimerisation and DNA binding property [1]. Thus, this family is probably related to Pfam:PF00170. This family also includes the DNA_binding domain of Skn-1 (Swiss:P34707), this domain lacks the leucine zipper found in other bZip domains, and binds DNA is a monomer [2,3].", "database": "PfamA", "aliStart": 139, "scoreName": "E-value", "accession": "PF03131.15", "start": 125, "score": 3.6e-05, "identifier": "bZIP Maf transcription factor", "type": "DBD", "aliEnd": 194}}], "length": 381}

{"regions": [{"startStyle": "jagged", "end": 49, "endStyle": "curved", "aliStart": 2, "text": "bZIP", "colour": "#009900", "aliEnd": 46, "start": 1, "href": "http://pfam.xfam.org/family/PF00170.19", "type": "pfama", "display": "true", "metadata": {"end": 49, "description": "The Pfam entry includes the basic region and the leucine zipper region.", "database": "PfamA", "aliStart": 2, "scoreName": "E-value", "accession": "PF00170.19", "start": 1, "score": 6.900000000000001e-06, "identifier": "bZIP transcription factor", "type": "DBD", "aliEnd": 46}}, {"startStyle": "jagged", "end": 52, "endStyle": "curved", "aliStart": 2, "text": "MAF", "colour": "#FFD800", "aliEnd": 46, "start": 1, "href": "http://pfam.xfam.org/family/PF03131.15", "type": "pfama", "display": "true", "metadata": {"end": 52, "description": "Maf transcription factors contain a conserved basic region leucine zipper (bZIP) domain, which mediates their dimerisation and DNA binding property [1]. Thus, this family is probably related to Pfam:PF00170. This family also includes the DNA_binding domain of Skn-1 (Swiss:P34707), this domain lacks the leucine zipper found in other bZip domains, and binds DNA is a monomer [2,3].", "database": "PfamA", "aliStart": 2, "scoreName": "E-value", "accession": "PF03131.15", "start": 1, "score": 0.0021, "identifier": "bZIP Maf transcription factor", "type": "DBD", "aliEnd": 46}}], "length": 233}

{"regions": [{"startStyle": "jagged", "end": 51, "endStyle": "jagged", "aliStart": 9, "text": "DUF3450", "colour": "#9999ff", "aliEnd": 46, "start": 4, "href": "http://pfam.xfam.org/family/PF11932.6", "type": "pfama", "display": "true", "metadata": {"end": 51, "description": "This family of proteins are functionally uncharacterised. This protein is found in bacteria and eukaryotes. Proteins in this family are about 260 amino acids in length.", "database": "PfamA", "aliStart": 9, "scoreName": "E-value", "accession": "PF11932.6", "start": 4, "score": 0.0068, "identifier": "Protein of unknown function (DUF3450)", "type": "DBD", "aliEnd": 46}}, {"startStyle": "jagged", "end": 50, "endStyle": "jagged", "aliStart": 6, "text": "IFT20", "colour": "#9999ff", "aliEnd": 44, "start": 3, "href": "http://pfam.xfam.org/family/PF14931.4", "type": "pfama", "display": "true", "metadata": {"end": 50, "description": "IFT20 is subunit 20 of the intraflagellar transport complex B [1]. The intraflagellar transport complex assembles and maintains eukaryotic cilia and flagella. IFT20 is localised to the Golgi complex and is anchored there by the Golgi polypeptide, GMAP210, whereas all other subunits except IFT172 localise to cilia and the peri-basal body or centrosomal region at the base of cilia [1,2,3].  IFT20 accompanies Golgi-derived vesicles to the point of exocytosis near the basal bodies where the other IFT polypeptides are present, and where the intact IFT particle is assembled in association with the inner surface of the cell membrane.  Passage of the IFT complex then follows, through the flagellar pore recognition site at the transition region, into the ciliary compartment.  There also appears to be a role of intraflagellar transport (IFT) polypeptides in the formation of the immune synapse in non ciliated cells. The flagellum, in addition to being a sensory and motile organelle, is also a secretory organelle [5]. A number of IFT components are expressed in haematopoietic cells, which have no cilia, indicating an unexpected role of IFT proteins in immune synapse-assembly and intracellular membrane trafficking in T lymphocytes; this suggests that the immune synapse could represent the functional homologue of the primary cilium in these cells [6,7].", "database": "PfamA", "aliStart": 6, "scoreName": "E-value", "accession": "PF14931.4", "start": 3, "score": 0.0095, "identifier": "Intraflagellar transport complex B, subunit 20", "type": "DBD", "aliEnd": 44}}], "length": 142}

{"regions": [{"startStyle": "curved", "end": 83, "endStyle": "curved", "aliStart": 21, "text": "bZIP", "colour": "#009900", "aliEnd": 80, "start": 21, "href": "http://pfam.xfam.org/family/PF00170.19", "type": "pfama", "display": "true", "metadata": {"end": 83, "description": "The Pfam entry includes the basic region and the leucine zipper region.", "database": "PfamA", "aliStart": 21, "scoreName": "E-value", "accession": "PF00170.19", "start": 21, "score": 3.7e-09, "identifier": "bZIP transcription factor", "type": "DBD", "aliEnd": 80}}, {"startStyle": "jagged", "end": 86, "endStyle": "curved", "aliStart": 25, "text": "MAF", "colour": "#FFD800", "aliEnd": 80, "start": 11, "href": "http://pfam.xfam.org/family/PF03131.15", "type": "pfama", "display": "true", "metadata": {"end": 86, "description": "Maf transcription factors contain a conserved basic region leucine zipper (bZIP) domain, which mediates their dimerisation and DNA binding property [1]. Thus, this family is probably related to Pfam:PF00170. This family also includes the DNA_binding domain of Skn-1 (Swiss:P34707), this domain lacks the leucine zipper found in other bZip domains, and binds DNA is a monomer [2,3].", "database": "PfamA", "aliStart": 25, "scoreName": "E-value", "accession": "PF03131.15", "start": 11, "score": 2e-05, "identifier": "bZIP Maf transcription factor", "type": "DBD", "aliEnd": 80}}], "length": 267}

TF Info Page for FOS (bZIP)