TF Info Page for MECP2 (MBD; AT hook)
Conclusion
| Assessment | Binding Mode | Motif Status | Notes | Comments |
|---|---|---|---|---|
| Known motif | 3 Low specificity DNA-binding protein | 100 perc ID - in vitro | Binds methylated CpG dinucleotides (PDB: 3C2I) |
Description
| Description: | methyl-CpG binding protein 2 [Source:HGNC Symbol;Acc:HGNC:6990] | ||||||||||||
| Entrez Summary | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015] | ||||||||||||
| Ensembl ID: | ENSG00000169057 | ||||||||||||
| External Link: | CisBP | ||||||||||||
| Interpro | IPR001739; IPR016177; IPR017353; ; | ||||||||||||
| Protein Domain: | ENSP00000301948 | ||||||||||||
| Protein Domain: | ENSP00000395535 | ||||||||||||
| Protein Domain: | ENSP00000486978 | ||||||||||||
| Protein Domain: | ENSP00000489847 | ||||||||||||
| Domain: |
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Previous Annotations
| Source | Annotation |
| TF-CAT classification |
TF Gene_Transcription Regulatory Activity heterochromatin interaction/binding_Non-specific DNA_ PMIDS:8177735 9038338 |
| Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources. |
x |
| CisBP considers it as a TF? | Yes |
| TFclass considers it as a TF? | No |
| Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" | No |
| GO-Info |
GO:0003700 sequence-specific DNA binding transcription factor activity IEA - GO_REF:0000019 |
|
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF |
3a, decent circumstantial evidence for its role as |
| TF has conditional DNA-binding requirements |