TF Info Page for STAT3 (STAT)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source

Description

Description: signal transducer and activator of transcription 3 [Source:HGNC Symbol;Acc:HGNC:11364]
Entrez Summary The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
Ensembl ID: ENSG00000168610
External Link: CisBP
Interpro IPR000980; IPR008967; IPR013799; IPR013800; IPR013801; IPR015988;
Protein Domain: ENSP00000264657
Protein Domain: ENSP00000384943
Protein Domain: ENSP00000467985
Protein Domain: ENSP00000373923
Domain:
Protein: ENSP00000264657DBD: STATOther: SH2, STAT_alpha, STAT_int
Protein: ENSP00000384943DBD: STATOther: SH2, STAT_alpha, STAT_int
Protein: ENSP00000467985DBD: STATOther: SH2, STAT_alpha, STAT_int
Protein: ENSP00000373923DBD: STATOther: SH2, STAT_alpha

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:8530402 10458605
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IMP - PMID:16946298
GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IDA - PMID:16835372
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:17344214
GO:0004879
ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity
IDA - PMID:17324931
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements Requires phosphorylation

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
ChIP-seqhmCHIPInferred - Stat3 (100% AA Identity, Mus musculus)
MiscHOMERInferred - Stat3 (100% AA Identity, Mus musculus)

Structure

Structure PDB 1BG1

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution