TF Info Page for PTF1A (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: pancreas specific transcription factor, 1a [Source:HGNC Symbol;Acc:HGNC:23734]
Entrez Summary This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000168267
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000365687
Domain:
Protein: ENSP00000365687DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:2294404 11279116
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
MiscHocoMocoDirect
MiscHOMERInferred - Ptf1a (100% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - Fer1 (86% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - FERD3L (60% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - FERD3L (60% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - FERD3L (60% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - FERD3L (60% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ATOH1 (51% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ATOH1 (51% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ATOH1 (51% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ATOH1 (51% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Atoh1 (51% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ATOH1 (51% AA Identity, Homo sapiens)
SELEXJASPARInferred - Atoh1 (51% AA Identity, Mus musculus)
MiscHOMERInferred - Atoh1 (51% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - amos (50% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - amos (50% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution