The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

recombination signal binding protein for immunoglobulin kappa J region [Source:HGNC Symbol;Acc:HGNC:

Entrez Summary

The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]

Ensembl ID:

ENSG00000168214

External Link:

CisBP

Interpro

IPR002909; IPR008967; IPR014756; IPR015350; IPR015351; ;

Protein Domain:

ENSP00000340124

Protein Domain:

ENSP00000305815

Protein Domain:

ENSP00000339699

Protein Domain:

ENSP00000347659

Protein Domain:

ENSP00000354528

Protein Domain:

ENSP00000423907

Protein Domain:

ENSP00000424804

Protein Domain:

ENSP00000423644

Protein Domain:

ENSP00000424459

Protein Domain:

ENSP00000424989

Domain:

Protein: ENSP00000340124	DBD: LAG1, DNA binding	Other: BTD, TIG
Protein: ENSP00000305815	DBD: LAG1, DNA binding	Other: BTD, TIG
Protein: ENSP00000339699	DBD: LAG1, DNA binding	Other: BTD, TIG
Protein: ENSP00000347659	DBD: LAG1, DNA binding	Other: BTD, TIG
Protein: ENSP00000354528	DBD: LAG1, DNA binding	Other: BTD, TIG
Protein: ENSP00000423907	DBD: LAG1, DNA binding	Other: BTD, TIG
Protein: ENSP00000424804	DBD: LAG1, DNA binding	Other:
Protein: ENSP00000423644	DBD: LAG1, DNA binding	Other:
Protein: ENSP00000424459	DBD: LAG1, DNA binding	Other:
Protein: ENSP00000424989	DBD: LAG1, DNA binding	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:8152928 8625826
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IDA - PMID:18663143 GO:0003700 sequence-specific DNA binding transcription factor activity TAS - PMID:9874765
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
COMPILED	JASPAR		Inferred - Su(H) (79% AA Identity, Drosophila melanogaster)
Transfac	Transfac	License required	Inferred - Su(H) (79% AA Identity, Drosophila melanogaster)
PBM	DelBianco2010		Direct
PBM	DelBianco2010		Direct
PBM	DelBianco2010		Direct
PBM	DelBianco2010		Direct
PBM	DelBianco2010		Direct
PBM	DelBianco2010		Direct
PBM	DelBianco2010		Direct
PBM	DelBianco2010		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct

Structure

Structure	PDB	2F8X

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for RBPJ (CSL)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution