The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Likely to be sequence specific TF	1 Monomer or homomultimer	No motif		Has the full GATA domain, and JPRED4 thinks the secondary structure is preserved. The GATA domain is not sufficiently well studied to declare that specific amino acids are absolutely required in order to bind DNA. Many unusual GATA domain proteins across eukaryotes bind DNA, making it reasonable to project that this one does, too.

Description

Description:	GATA zinc finger domain containing 2A [Source:HGNC Symbol;Acc:HGNC:29989]
Entrez Summary	TBA
Ensembl ID:	ENSG00000167491
External Link:
Interpro	IPR000679; ;
Protein Domain:
Protein: ENSP00000351552	DBD: GATA	Other: P66_CC
Protein: ENSP00000384899	DBD: GATA	Other: P66_CC
Protein: ENSP00000403703	DBD: GATA	Other: P66_CC
Protein: ENSP00000404212	DBD: GATA	Other: P66_CC
Protein: ENSP00000477171	DBD: GATA	Other:
Protein: ENSP00000414252	DBD: GATA	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	No PMIDS:
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	x
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	No
GO-Info	GO:0003700 sequence-specific DNA binding transcription factor activity IEA - GO_REF:0000002
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	4a, two or more datasets predict it as a TF
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 451, "endStyle": "curved", "aliStart": 417, "text": "GATA", "colour": "#2cb42c", "aliEnd": 450, "start": 417, "href": "http://pfam.xfam.org/family/PF00320.25", "type": "pfama", "display": "true", "metadata": {"end": 451, "description": "This domain uses four cysteine residues to coordinate a zinc ion. This domain binds to DNA. Two GATA zinc fingers are found in the GATA transcription factors.  However there are several proteins which only contain a single copy of the domain.", "database": "PfamA", "aliStart": 417, "scoreName": "E-value", "accession": "PF00320.25", "start": 417, "score": 2.2e-07, "identifier": "GATA zinc finger", "type": "DBD", "aliEnd": 450}}, {"startStyle": "straight", "end": 179, "endStyle": "straight", "aliStart": 137, "text": "P66_CC", "colour": "#9999ff", "aliEnd": 179, "start": 136, "href": "http://pfam.xfam.org/family/PF16563.3", "type": "pfama", "display": "true", "metadata": {"end": 179, "description": "This family is a short coiled-coil interaction region on the transcriptional repressors P66A and P66B. The P66A and B, or alpha and beta, complex with MBDs or methyl-binding domain-containing proteins via a coiled-coil region on each. This P66-MBD2 complex forms part of an assembly with NuRD, nucleosome remodelling and deacetylation protein.  MBD2-NuRD binds methylated DNA and regulates transcription of eg, the foetal beta-globin gene during development [1].", "database": "PfamA", "aliStart": 137, "scoreName": "E-value", "accession": "PF16563.3", "start": 136, "score": 2.4e-20, "identifier": "Coiled-coil and interaction region of P66A and P66B with MBD2", "type": "DBD", "aliEnd": 179}}], "length": 634}

{"regions": [{"startStyle": "curved", "end": 452, "endStyle": "curved", "aliStart": 418, "text": "GATA", "colour": "#2cb42c", "aliEnd": 451, "start": 418, "href": "http://pfam.xfam.org/family/PF00320.25", "type": "pfama", "display": "true", "metadata": {"end": 452, "description": "This domain uses four cysteine residues to coordinate a zinc ion. This domain binds to DNA. Two GATA zinc fingers are found in the GATA transcription factors.  However there are several proteins which only contain a single copy of the domain.", "database": "PfamA", "aliStart": 418, "scoreName": "E-value", "accession": "PF00320.25", "start": 418, "score": 2.2e-07, "identifier": "GATA zinc finger", "type": "DBD", "aliEnd": 451}}, {"startStyle": "straight", "end": 179, "endStyle": "straight", "aliStart": 137, "text": "P66_CC", "colour": "#9999ff", "aliEnd": 179, "start": 136, "href": "http://pfam.xfam.org/family/PF16563.3", "type": "pfama", "display": "true", "metadata": {"end": 179, "description": "This family is a short coiled-coil interaction region on the transcriptional repressors P66A and P66B. The P66A and B, or alpha and beta, complex with MBDs or methyl-binding domain-containing proteins via a coiled-coil region on each. This P66-MBD2 complex forms part of an assembly with NuRD, nucleosome remodelling and deacetylation protein.  MBD2-NuRD binds methylated DNA and regulates transcription of eg, the foetal beta-globin gene during development [1].", "database": "PfamA", "aliStart": 137, "scoreName": "E-value", "accession": "PF16563.3", "start": 136, "score": 2.5e-20, "identifier": "Coiled-coil and interaction region of P66A and P66B with MBD2", "type": "DBD", "aliEnd": 179}}], "length": 635}

TF Info Page for GATAD2A (GATA)