TF Info Page for SP2 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Sp2 transcription factor [Source:HGNC Symbol;Acc:HGNC:11207]
Entrez Summary This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000167182
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000365931
Protein Domain: ENSP00000489736
Protein Domain: ENSP00000490512
Protein Domain: ENSP00000490351
Domain:
Protein: ENSP00000365931DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000489736DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000490512DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000490351DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
RCADESchmitges2016Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
ChIP-seqENCODEDirect
ChIP-seqJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - Sp4 (78% AA Identity, Mus musculus)
SMiLE-seqIsakova2017Inferred - Sp4 (78% AA Identity, Mus musculus)
PBMZoo_01Inferred - Sp4 (78% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - Sp1 (76% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - SP3 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP3 (76% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - SP8 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP8 (76% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - SP9 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP9 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP3 (76% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - SP4 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP4 (76% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP3 (76% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP4 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP5 (75% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Sp1 (73% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - SP1 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP1 (72% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - SP1 (72% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - SP1 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (72% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP1 (72% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP1 (72% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP7 (71% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution