TF Info Page for MBD6 (MBD)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Likely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Has not been tested on PBM or HT-SELEX. Some evidence exists showing that it does not bind methylated DNA (PMID: 20700456), and the MBD domain instead facilitates protein interactions (PMID: 24634419).

Description

Description: methyl-CpG binding domain protein 6 [Source:HGNC Symbol;Acc:HGNC:20445]
Entrez Summary NA
Ensembl ID: ENSG00000166987
External Link: CisBP
Interpro IPR001739; IPR016177;
Protein Domain: ENSP00000347896
Protein Domain: ENSP00000448202
Protein Domain: ENSP00000450160
Protein Domain: ENSP00000446834
Domain:
Protein: ENSP00000347896DBD: Methyl-CpG DNA-bindingOther:
Protein: ENSP00000448202DBD: Methyl-CpG DNA-bindingOther:
Protein: ENSP00000450160DBD: Methyl-CpG DNA-bindingOther:
Protein: ENSP00000446834DBD: Methyl-CpG DNA-bindingOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
No
CisBP considers it as a TF? Yes
TFclass considers it as a TF? No
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
5a, one of the source datasets predicts is as a TF
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution