TF Info Page for SMAD3 (SMAD)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: SMAD family member 3 [Source:HGNC Symbol;Acc:HGNC:6769]
Entrez Summary The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
Ensembl ID: ENSG00000166949
External Link: CisBP
Interpro IPR001132; IPR003619; IPR008984; IPR013019;
Protein Domain: ENSP00000332973
Protein Domain: ENSP00000401133
Protein Domain: ENSP00000445348
Protein Domain: ENSP00000437757
Protein Domain: ENSP00000453684
Protein Domain: ENSP00000455540
Domain:
Protein: ENSP00000332973DBD: MH1 Family DBDsOther: IRF-3, MH2
Protein: ENSP00000401133DBD: MH1 Family DBDsOther: IRF-3, MH2
Protein: ENSP00000445348DBD: MH1 Family DBDsOther: IRF-3, MH2
Protein: ENSP00000437757DBD: MH1 Family DBDsOther: IRF-3, MH2
Protein: ENSP00000453684DBD: MH1 Family DBDsOther:
Protein: ENSP00000455540DBD: MH1 Family DBDsOther: MH2

Previous Annotations

Source Annotation
TF-CAT classification TF Gene Candidate_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:9660945
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000983
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity
IDA - PMID:21947082 | IMP - PMID:26311719
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:10823886, PMID:21947082, PMID:9111321,
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - Smad3 (100% AA Identity, Mus musculus)
MiscHOMERInferred - Smad3 (100% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - SMAD2 (70% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SMAD2 (70% AA Identity, Homo sapiens)
MiscHOMERInferred - SMAD2 (70% AA Identity, Homo sapiens)

Structure

Structure PDB 1MHD

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution