TF Info Page for GFI1B (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: growth factor independent 1B transcriptional repressor [Source:HGNC Symbol;Acc:HGNC:4238]
Entrez Summary This gene encodes a zinc-finger containing transcriptional regulator that is primarily expressed in cells of hematopoietic lineage. The encoded protein complexes with numerous other transcriptional regulatory proteins including GATA-1, runt-related transcription factor 1 and histone deacetylases to control expression of genes involved in the development and maturation of erythrocytes and megakaryocytes. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet-type bleeding disorder-17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
Ensembl ID: ENSG00000165702
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000344782
Protein Domain: ENSP00000361195
Protein Domain: ENSP00000490303
Protein Domain: ENSP00000489646
Domain:
Protein: ENSP00000344782DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000361195DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000490303DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000489646DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
PBMBarrera2016Direct
PBMBarrera2016Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
ChIP-seqJASPARInferred - Gfi1b (99% AA Identity, Mus musculus)
MiscHOMERInferred - Gfi1b (99% AA Identity, Mus musculus)
PBMBarrera2016Inferred - GFI1 (87% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - GFI1 (87% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - GFI1 (87% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - GFI1 (87% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - GFI1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GFI1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GFI1 (87% AA Identity, Homo sapiens)
SELEXJASPARInferred - Gfi1 (87% AA Identity, Rattus norvegicus)
MiscHocoMocoInferred - GFI1 (87% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - sens (81% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution