The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

hes related family bHLH transcription factor with YRPW motif 1 [Source:HGNC Symbol;Acc:HGNC:4880]

Entrez Summary

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Ensembl ID:

ENSG00000164683

External Link:

CisBP

Interpro

IPR003650; IPR011598;

Protein Domain:

ENSP00000338272

Protein Domain:

ENSP00000346761

Protein Domain:

ENSP00000429792

Protein Domain:

ENSP00000429705

Domain:

Protein: ENSP00000338272	DBD: bHLH	Other: Hairy_orange
Protein: ENSP00000346761	DBD: bHLH	Other: Hairy_orange
Protein: ENSP00000429792	DBD: bHLH	Other: Hairy_orange
Protein: ENSP00000429705	DBD: bHLH	Other: Hairy_orange

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene Candidate_Transcription Factor Binding tf co-factor binding_Transactivation_ PMIDS:16199874
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0000983 RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity ISS - GO_REF:0000024 GO:0003700 sequence-specific DNA binding transcription factor activity IDA - PMID:15485867
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Inferred - HEY2 (88% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - HEY2 (88% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - HEY2 (88% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - HEY2 (88% AA Identity, Homo sapiens)
PBM	Zoo_01		Inferred - Hey2 (88% AA Identity, Mus musculus)
Misc	HocoMoco		Inferred - HEY2 (88% AA Identity, Homo sapiens)
HT-SELEX	Nitta2015		Inferred - Hey (75% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - Hey (75% AA Identity, Drosophila melanogaster)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for HEY1 (bHLH)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution