TF Info Page for HAND2 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: heart and neural crest derivatives expressed 2 [Source:HGNC Symbol;Acc:HGNC:4808]
Entrez Summary The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000164107
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000352565
Protein Domain: ENSP00000477638
Domain:
Protein: ENSP00000352565DBD: bHLHOther:
Protein: ENSP00000477638DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding
DNA-Binding
sequence-specific_TF PPI
Transactivation_
PMIDS:11812799 12392994
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:15486975
GO:0043433
negative regulation of sequence-specific DNA binding transcription factor activity
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
MiscHocoMocoInferred - HAND1 (88% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - BHLHA15 (54% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - BHLHA15 (54% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHA15 (54% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHA15 (54% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Bhlha15 (54% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - TAL1 (54% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TAL1 (54% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TAL1 (54% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TAL1 (54% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TAL1 (54% AA Identity, Homo sapiens)
MiscHOMERInferred - Tal1 (54% AA Identity, Mus musculus)
MiscHOMERInferred - Tal1 (54% AA Identity, Mus musculus)
PBMZoo_01Inferred - Twist2 (51% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - TWIST1 (51% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TWIST1 (51% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TWIST1 (51% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ATOH7 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ATOH7 (50% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - dimm (50% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - dimm (50% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - NHLH1 (50% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NHLH1 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NHLH1 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NHLH1 (50% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NHLH2 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NHLH2 (50% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Nhlh2 (50% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - twi (50% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - twi (50% AA Identity, Drosophila melanogaster)
SELEXJASPARInferred - NHLH1 (50% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NHLH1 (50% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NHLH1 (50% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Nhlh2 (50% AA Identity, Mus musculus)
DNaseI footprintingJASPARInferred - twi (50% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - twi (50% AA Identity, Drosophila melanogaster)
MiscHocoMocoInferred - NHLH1 (50% AA Identity, Homo sapiens)
MisciDMMPMMInferred - twi (50% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution