TF Info Page for HEYL (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Inferred motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: hes related family bHLH transcription factor with YRPW motif-like [Source:HGNC Symbol;Acc:HGNC:4882]
Entrez Summary This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000163909
External Link: CisBP
Interpro IPR003650; IPR011598;
Protein Domain: ENSP00000361943
Domain:
Protein: ENSP00000361943DBD: bHLHOther: Hairy_orange

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000983
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity
ISS - GO_REF:0000024
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:15485867
GO:2000824
negative regulation of androgen receptor activity
IDA - PMID:21454491
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a2, Orthologous HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Inferred - HEY2 (91% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HEY2 (91% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HEY2 (91% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HEY2 (91% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Hey2 (91% AA Identity, Mus musculus)
MiscHocoMocoInferred - HEY2 (91% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HEY1 (83% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - HEY1 (83% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HEY1 (83% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HEY1 (83% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - Hey (76% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Hey (76% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution