The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	nuclear factor I A [Source:HGNC Symbol;Acc:HGNC:7784]
Entrez Summary	TBA
Ensembl ID:	ENSG00000162599
External Link:	T141886_1.02
Interpro	IPR000647; IPR003619; IPR019548; IPR019739; IPR020604; ;
Protein Domain:
Protein: ENSP00000360229	DBD: MH1 Family DBDs	Other: CTF_NFI, NfI_DNAbd_pre-N
Protein: ENSP00000384523	DBD: MH1 Family DBDs	Other: CTF_NFI, NfI_DNAbd_pre-N
Protein: ENSP00000474817	DBD: MH1 Family DBDs	Other: CTF_NFI
Protein: ENSP00000474462	DBD: MH1 Family DBDs	Other: CTF_NFI
Protein: ENSP00000360231	DBD: MH1 Family DBDs	Other: CTF_NFI, NfI_DNAbd_pre-N
Protein: ENSP00000384680	DBD: MH1 Family DBDs	Other: CTF_NFI, NfI_DNAbd_pre-N
Protein: ENSP00000474461	DBD: MH1 Family DBDs	Other: CTF_NFI
Protein: ENSP00000474806	DBD: MH1 Family DBDs	Other: CTF_NFI
Protein: ENSP00000473830	DBD: MH1 Family DBDs	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding TF PPI Transactivation_ PMIDS:16169882 17010934
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IDA - PMID:17010934 GO:0003700 sequence-specific DNA binding transcription factor activity NAS - PMID:7590749
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2013		Direct
HT-SELEX	Jolma2013		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Inferred - Nfia (100% AA Identity, Rattus norvegicus)
HT-SELEX	Yin2017		Inferred - NFIX (94% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - NFIX (94% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - NFIB (92% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - NFIB (92% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - NFIB (92% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - NFIC (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - NFIC (89% AA Identity, Homo sapiens)
High-throughput SELEX SAGE	JASPAR		Inferred - NFIC (89% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - NFIC (89% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - NFIC (89% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - NFIC (89% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - NFIC (89% AA Identity, Homo sapiens)
HT-SELEX	Nitta2015		Inferred - NfI (80% AA Identity, Drosophila melanogaster)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for NFIA (SMAD)