TF Info Page for IKZF3 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro ChIP-seq motif is consistent with recognition code (RCADE)

Description

Description: IKAROS family zinc finger 3 [Source:HGNC Symbol;Acc:HGNC:13178]
Entrez Summary This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012]
Ensembl ID: ENSG00000161405
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000344544
Protein Domain: ENSP00000367179
Protein Domain: ENSP00000367180
Protein Domain: ENSP00000367188
Protein Domain: ENSP00000367194
Protein Domain: ENSP00000377741
Protein Domain: ENSP00000403776
Protein Domain: ENSP00000438972
Protein Domain: ENSP00000485515
Protein Domain: ENSP00000462452
Domain:
Protein: ENSP00000344544DBD: C2H2 ZF Containing ProteinsOther: zf-C2H2_4
Protein: ENSP00000367179DBD: C2H2 ZF Containing ProteinsOther: zf-C2H2_4
Protein: ENSP00000367180DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000367188DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000367194DBD: C2H2 ZF Containing ProteinsOther: zf-C2H2_4
Protein: ENSP00000377741DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000403776DBD: C2H2 ZF Containing ProteinsOther: zf-C2H2_4
Protein: ENSP00000438972DBD: C2H2 ZF Containing ProteinsOther: zf-C2H2_4
Protein: ENSP00000485515DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000462452DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding
DNA-Binding
sequence-specific_DNA Binding
TF PPI
Transactivation_
PMIDS:9155026
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:10552935
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
RCADESchmitges2016Direct
TransfacTransfacLicense requiredInferred - IKZF1 (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IKZF1 (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IKZF1 (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IKZF1 (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IKZF1 (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IKZF1 (81% AA Identity, Homo sapiens)
MiscHocoMocoInferred - IKZF1 (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IKZF2 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IKZF2 (78% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution