TF Info Page for ARHGAP35 (Unknown)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Likely to be sequence specific TF 1 Monomer or homomultimer No motif Binds DNA specifically based on EMSA and nuclease protection assay data (PMID: 1894621), despite lacking any clear DBDs.

Description

Description: Rho GTPase activating protein 35 [Source:HGNC Symbol;Acc:HGNC:4591]
Entrez Summary The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000160007
External Link: CisBP
Interpro IPR000198; IPR001806; IPR002713; IPR008936; IPR027417; ;
Protein Domain: ENSP00000385720
Protein Domain: ENSP00000483730
Protein Domain: ENSP00000479487
Domain:
Protein: ENSP00000385720DBD: OtherOther: FF, Ras, RhoGAP, RhoGAP-FF1
Protein: ENSP00000483730DBD: OtherOther: FF, Ras, RhoGAP, RhoGAP-FF1
Protein: ENSP00000479487DBD: OtherOther: FF, Ras, RhoGAP-FF1

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:1894621
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
other
CisBP considers it as a TF? No
TFclass considers it as a TF? No
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001227
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IDA - PMID:1894621
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
4a, two or more datasets predict it as a TF
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution