TF Info Page for SIM2 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Inferred motif 2 Obligate heteromer In vivo/Misc source Likely obligate hetereomer (PMID: 7592839)

Description

Description: single-minded family bHLH transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:10883]
Entrez Summary This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Ensembl ID: ENSG00000159263
External Link: CisBP
Interpro IPR000014; IPR001610; IPR010578; IPR011598; IPR013655; IPR013767;
Protein Domain: ENSP00000290399
Protein Domain: ENSP00000392003
Protein Domain: ENSP00000476273
Domain:
Protein: ENSP00000290399DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_9, SIM_C
Protein: ENSP00000392003DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9, SIM_C
Protein: ENSP00000476273DBD: bHLHOther: PAS, PAS_11, PAS_3

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
non-sequence-specific
Transcription Factor Binding
tf co-factor binding_Co-activation_
PMIDS:11782478
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
other
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:8661114, PMID:9199934
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a2, Orthologous HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredInferred - HIF1A (58% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (58% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (58% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (58% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (58% AA Identity, Homo sapiens)
MiscHocoMocoInferred - HIF1A (58% AA Identity, Homo sapiens)
MiscHOMERInferred - HIF1A (58% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - EPAS1 (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - EPAS1 (55% AA Identity, Homo sapiens)
MiscHocoMocoInferred - EPAS1 (55% AA Identity, Homo sapiens)
MiscHOMERInferred - EPAS1 (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Hif3a (50% AA Identity, Mus musculus)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution