TF Info Page for USF1 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: upstream transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12593]
Entrez Summary This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. [provided by RefSeq, Feb 2013]
Ensembl ID: ENSG00000158773
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000356999
Protein Domain: ENSP00000357000
Protein Domain: ENSP00000433041
Domain:
Protein: ENSP00000356999DBD: bHLHOther:
Protein: ENSP00000357000DBD: bHLHOther:
Protein: ENSP00000433041DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:9677331
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
IDA - PMID:2249772 | IMP - PMID:18234320, PMID:857
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERDirect
PBMZoo_01Inferred - Usf1 (100% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - USF2 (85% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - USF2 (85% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Usf2 (85% AA Identity, Mus musculus)
ChIP-seqENCODEInferred - USF2 (85% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - USF2 (85% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - USF2 (85% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - USF2 (85% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - USF2 (85% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - USF2 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - USF2 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - USF2 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - USF2 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - USF2 (85% AA Identity, Homo sapiens)
MiscHocoMocoInferred - USF2 (85% AA Identity, Homo sapiens)
MiscHOMERInferred - Usf2 (85% AA Identity, Mus musculus)

Structure

Structure PDB 1AN4

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution