TF Info Page for FLI1 (Ets)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Fli-1 proto-oncogene, ETS transcription factor [Source:HGNC Symbol;Acc:HGNC:3749]
Entrez Summary This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Ensembl ID: ENSG00000151702
External Link: CisBP
Interpro IPR000418; IPR003118; IPR013761; ;
Protein Domain: ENSP00000281428
Protein Domain: ENSP00000433488
Protein Domain: ENSP00000339627
Protein Domain: ENSP00000432950
Domain:
Protein: ENSP00000281428DBD: ETSOther: SAM_PNT
Protein: ENSP00000433488DBD: ETSOther: SAM_PNT
Protein: ENSP00000339627DBD: ETSOther:
Protein: ENSP00000432950DBD: ETSOther: SAM_PNT

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_Transactivation_
PMIDS:1445800 1522903 8449942 8502483 12065417 14570912
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:10891501
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
SMiLE-seqIsakova2017Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
SELEXJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMWei10Inferred - Fli1 (100% AA Identity, Mus musculus)
MiscHOMERInferred - Fli1 (100% AA Identity, Mus musculus)
HT-SELEXJolma2010Inferred - ERG (97% AA Identity, Homo sapiens)
HT-SELEXJolma2010Inferred - ERG (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ERG (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ERG (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ERG (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ERG (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ERG (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ERG (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ERG (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ERG (97% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - Ets65A (97% AA Identity, Drosophila melanogaster)
SELEXJASPARInferred - ERG (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ERG (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ERG (97% AA Identity, Homo sapiens)
MiscHocoMocoInferred - ERG (97% AA Identity, Homo sapiens)
MiscHOMERInferred - ERG (97% AA Identity, Homo sapiens)
PBMWei10Inferred - Erg (96% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - FEV (88% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - FEV (88% AA Identity, Homo sapiens)
MiscHocoMocoInferred - FEV (88% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - Ets21C (86% AA Identity, Drosophila melanogaster)
PBMZoo_01Inferred - Ets21C (86% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - pnt (64% AA Identity, Drosophila melanogaster)
PBMWei10Inferred - Ets1 (63% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ETS1B_CHICK (63% AA Identity, Gallus gallus)
HT-SELEXYin2017Inferred - ETV5 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV5 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV5 (58% AA Identity, Homo sapiens)
PBMWei10Inferred - Etv5 (58% AA Identity, Mus musculus)
MiscHocoMocoInferred - ETV5 (58% AA Identity, Homo sapiens)
PBMZoo_01Inferred - elk4 (55% AA Identity, Tetraodon nigroviridis)
HT-SELEXNitta2015Inferred - Ets96B (55% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - ETV1 (55% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ETV1 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV1 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV1 (55% AA Identity, Homo sapiens)
PBMWei10Inferred - Etv1 (55% AA Identity, Mus musculus)
PBMWei10Inferred - Etv1 (55% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - ETV4 (55% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ETV4 (55% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ETV4 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV4 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV4 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV4 (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV4 (55% AA Identity, Homo sapiens)
PBMWei10Inferred - Etv4 (55% AA Identity, Mus musculus)
SMiLE-seqIsakova2017Inferred - GABPA (55% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - GABPA (55% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - GABPA (55% AA Identity, Homo sapiens)
PBMBadis09Inferred - Gabpa (55% AA Identity, Mus musculus)
PBMWei10Inferred - Gabpa (55% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ETV4 (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ETV4 (55% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - GABPA (55% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - GABPA (55% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - GABPA (55% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - GABPA (55% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - GABPA (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GABPA (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GABPA (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GABPA (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GABPA (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GABPA (55% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GABPA (55% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - Gabpa (55% AA Identity, Mus musculus)
MiscHOMERInferred - ETV1 (55% AA Identity, Homo sapiens)
MiscHocoMocoInferred - ETV4 (55% AA Identity, Homo sapiens)
MiscHocoMocoInferred - GABPA (55% AA Identity, Homo sapiens)
MiscHOMERInferred - GABPA (55% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ELK1 (54% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ELK1 (54% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ELK1 (54% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ELK1 (54% AA Identity, Homo sapiens)
PBMWei10Inferred - Elk1 (54% AA Identity, Mus musculus)
SELEXJASPARInferred - ELK1 (54% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ELK1 (54% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ELK1 (54% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ELK1 (54% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ELK1 (54% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ELK1 (54% AA Identity, Homo sapiens)
MiscHocoMocoInferred - ELK1 (54% AA Identity, Homo sapiens)
MiscHOMERInferred - ELK1 (54% AA Identity, Homo sapiens)
PBMWei10Inferred - Elk4 (53% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - ETV2 (53% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV2 (53% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ETV3 (53% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ETV3 (53% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV3 (53% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ETV3 (53% AA Identity, Homo sapiens)
PBMWei10Inferred - Etv3 (53% AA Identity, Mus musculus)
PBMWei10Inferred - XP_911724.4 (53% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ETV2 (53% AA Identity, Homo sapiens)

Structure

Structure PDB 5E8I

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution